Virginia iGEM Workshop #2 High School Education Series

Workshop Plan Virginia iGEM Lesson: Genes, Backbones, and BioBrick Structure Case Study: FtsZ and Minicell Formation (VGEM 2013) Activity: DNA Extraction http://parts.igem.org/Main_Page Workshop #2 High School Education Series Virginia iGEM

Genes Virginia iGEM Workshop #2 High School Education Series http://www.dnarss.com/Chromosome_1.html http://www.fragilex.org/fragile-x-associated-disorders/genetics-and-inheritance/fmr1-gene/ Workshop #2 High School Education Series Virginia iGEM

Promoters and Repressors Genetic Toggle Switch Repressilator Workshop #2 High School Education Series Virginia iGEM For more information, see: Khalil, A. S., & Collins, J. J. (2010). Synthetic biology: Applications come of age. Nature Reviews Genetics, 11, 367-379.

Gene Construct Structure Ribosome Binding Site Promoter RBS Gene Terminator Green Fluorescent Protein Promoter RBS Gene RBS GFP Terminator (Reporter Gene) Workshop #2 High School Education Series Virginia iGEM

Fluorescence for fun… 

for science…

Plasmids Virginia iGEM Workshop #2 High School Education Series http://en.wikipedia.org/wiki/Plasmid http://www.etap.org/demo/biology_files/lesson5/instructiontutor_last.html Electron Micrograph of Ruptured E. Coli Cell Workshop #2 High School Education Series Virginia iGEM

Plasmid Backbone Virginia iGEM GENE Workshop #2 High School Education Series Virginia iGEM

Restriction Enzymes Virginia iGEM Workshop #2 Sticky Ends http://www.bio.miami.edu/dana/pix/stickyends.jpg Workshop #2 High School Education Series Virginia iGEM

Case Study: FtsZ and Minicell Formation (VGEM 2013) Z-Ring Off-Center http://schaechter.asmblog.org/schaechter/2007/07/minicells-on--1.html Workshop #2 High School Education Series Virginia iGEM

Activity: DNA Extraction http://parts.igem.org/Main_Page Workshop #2 High School Education Series Virginia iGEM

Any Questions? Virginia iGEM Workshop #2 High School Education Series http://www.padillabay.gov/brant/ask.html Workshop #2 High School Education Series Virginia iGEM