1. Moving Green Fluorescent Protein
A jellyfish gene is identified that codes for a protein (GFP) that glows under UV light.
Researchers clone the gene and insert it into bacteria.

2. Green Fluorescent Protein
Plasmid from E. coli
amp resistance
ara B
ara A
ara D P
Green Fluorescent Protein O
ara C
Gene that codes for the inhibitor protein
Operator for the arabinose operon.
Gene that gives bacteria resistance to the antibiotic ampicillin.
Promoter for the arabinose operon.
Structural genes for arabinose metabolism

3. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
Repressor protein
(from ara C gene) O P
GREEN FLUOESCENT PROTEIN
GFP gene
(from jellyfish)
Regulatory genes
(operator & promoter)

4. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
RNA
polymerase O P
GREEN FLUOESCENT PROTEIN

5. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
Arabinose O P
GREEN FLUOESCENT PROTEIN

6. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
Arabinose O P
GREEN FLUOESCENT PROTEIN

7. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
Arabinose
Arabinose O P
GREEN FLUOESCENT PROTEIN

8. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
RNA
polymerase
Arabinose O P
GREEN FLUOESCENT PROTEIN

9. Function of the GFP gene in pGLO GREEN FLUOESCENT PROTEIN
RNA
polymerase
Arabinose O P
mRNA
GREEN FLUOESCENT PROTEIN