Immunologic responses after the MN-mediated cancer immunotherapy.

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Immunologic responses after the MN-mediated cancer immunotherapy. Immunologic responses after the MN-mediated cancer immunotherapy. (A) Representative quantitative analysis of T cells (gated on CD3+ T cells) in treated tumors analyzed by flow cytometry. (B) Representative quantitative analysis of activated DCs (CD86+ and CD80+) in the draining lymph nodes analyzed by flow cytometry. Data points represent mean ± SD (n = 8). (C) Immunofluorescence staining of the tumors showing CD4+ T cell and CD8+ T cell infiltration (scale bars, 100 μm). (D) Quantification of IgG1 subtypes in serum collected at day 10. Data points represent mean ± SD (n = 8). (E) Cytotoxic responses of splenocytes against B16F10 cells in vitro. Data points represent mean ± SD (n = 6). (F) Immunofluorescence staining of HSP70 (green) in the regional skin with actin filaments visualized by Alexa Fluor 660 phalloidin (red) and cell nuclei stained with Hoechst (blue) (scale bars, 100 μm). (G) In vivo local detection of cytokines from extracted patches at day 3. Statistical significance was calculated by Student’s t test (*P < 0.05; **P < 0.01). Data points represent mean ± SD (n = 8). Error bars indicate SD. CTL, cytotoxic T lypmphocyte. Yanqi Ye et al. Sci. Immunol. 2017;2:eaan5692 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works