Tuning lipid mixtures to induce or suppress domain formation across leaflets of unsupported asymmetric bilayers Marcus D. Collins and Sarah L. Keller.

Slides:

Advertisements

Similar presentations

Ways to separate mixtures – Chapter 3: Matter & Its Properties

Advertisements

Results from a field-theoretic approach to membrane fusion Michael Schick Department of Physics University of Washington.

Investigation: Oil and Water (cell membrane)

Surface-supported Bilayer Lipid Membranes from Lipid Mixtures: Composition and Transverse Molecular Flip-Flop Prabhanshu Shekhar 1, Frank Heinrich 1,2,

Third Generation Fuel Induction Systems for S.I. Engines

Do Now Write a few sentences to describe the characteristics of magnets that you know?

Mixtures of Matter. Classification of Matter.

The effect of resorcinolic lipids on biological membranes Magdalena Siwko.

 decimals/cc-7th-fracs-to-decimals/v/converting-fractions-to-decimals-example.

3.3 Cell Membrane KEY CONCEPT The cell membrane is a barrier that separates a cell from the external environment.

The Electricity and Magnetism Connection

+ Separation Methods. + Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties.

DNA Technology. There are currently many types of Biotechnology that are being used across the world What does Biotechnology mean???

Chemistry Chapter 3 Notes #2. Matter Pure Substances (can be represented by a chemical Formula) ElementsCompounds Mixtures (can be separated By physical.

Periodic Table Trends. (1) Atomic Radius As one goes from left to right across a period, the atomic radii decreases. Reason: All the elements in a period.

Domain Registration in Raft-Mimicking Lipid Mixtures Studied Using Polymer-Tethered Lipid Bilayers Sumit Garg, Jürgen Rühe, Karin Lüdtke, Rainer Jordan,

Phospholipid A phospholipid is a type of lipid used in the cells of living things.

Percentage without a calculator 10% 10%, 20 %, 30% …. 90% 25%, 50% or 75% 33 ⅓ % and 66 ⅔ % 5% 15%, 25%, 35% etc Mixture.

Potential Driven Flows Through Bifurcating Networks Aerospace and Mechanical Engineering Graduate Student Conference October, 2006 Jason Mayes.

3.3 Cell Membrane TEKS 3E, 4B, 9A The student is expected to: 3E evaluate models according to their limitations in representing biological objects or events;

MIXTURES AND COMPOUNDS. Types of Mixtures Two types of mixtures exist 1. Homogeneous Uniform in nature Cannot tell that it is a mixture by just looking.

General, Organic, and Biological Chemistry Copyright © 2010 Pearson Education, Inc.1 Chapter 17 Lipids 17.8 Cell Membranes.

SEPARATING MIXTURES Chapter 3. SEPARATING MIXTURES Because substances in a mixture are physically combined, the processes used to separate a mixture are.

+ Separation Methods Ways to separate mixtures. + Separating Mixtures Substances in a mixture are physically combined, so processes based on differences.

Homeostasis and Cellular Transport. Cell Membrane A phospholipid bilayer that forms the outer membrane of a cell Is selectively permeable Controls which.

The separation of a mixture of insoluble solids Critical Question: How can a mixture of two solids that are insoluble in water be separated?

Compounds revisited: A substance composed of two or more elements that are chemically combined.

Tables and Graphs Tables : organizes and displays data Line graph: shows how something has changed over a period of time. Example: Polar bear’s level of.

Objectives: 1. Define and calculate the capacitance of a capacitor. 2. Describe the factors affecting the capacitance of the capacitor. 3. Calculate the.

Background information Light is known to be an essential part on photosynthesis. Different forms of light (natural/ artificial)achieve the same result.

Pure Substances and Mixtures. Pure Substances Elements cannot be broken down chemically into anything else – they are pure substances. Compounds can be.

The student is expected to:

Selective Breeding Limitations of selective breeding and mutations:

Cell Physiol Biochem 2013;32: DOI: /

Introduction Intro Problem Materials Hypothesis Procedure Results

Separation Methods.

Cell Structure and Function 7.2

Used for composite functions

The Structure and Function of the Plasma Membrane

The Effects of Poster Format on Reading Time

The student is expected to:

פחת ורווח הון סוגיות מיוחדות תהילה ששון עו"ד (רו"ח) ספטמבר 2015

Combinations of Functions; Composite Functions

التعلم بالإكتشاف المراجع:

The Effects of Poster Format on Reading Time

Separation Methods: Review of Mixtures

The student is expected to:

Adding Specificity to Artificial Transcription Activators

Activity 3: Plan to Separate the Mixture

Do Now What is the role of the cell membrane?

Composition Fluctuations in Lipid Bilayers

Nanostructure of Cationic Lipid-Oligonucleotide Complexes

A B Combined consent and compound HIPAA authorization

H.M. Seeger, G. Marino, A. Alessandrini, P. Facci Biophysical Journal

Paper Presentation 25th April 2014 Rijeesh K.

Obstructed Diffusion in Phase-Separated Supported Lipid Bilayers: A Combined Atomic Force Microscopy and Fluorescence Recovery after Photobleaching Approach

G. Garbès Putzel, Mark J. Uline, Igal Szleifer, M. Schick

Molecular Model of a Cell Plasma Membrane With an Asymmetric Multicomponent Composition: Water Permeation and Ion Effects Robert Vácha, Max L. Berkowitz,

A B Combined consent and compound HIPAA authorization

Acyl Chain Length and Saturation Modulate Interleaflet Coupling in Asymmetric Bilayers: Effects on Dynamics and Structural Order Salvatore Chiantia,

On the Role of Acylation of Transmembrane Proteins

The student is expected to:

Classifying Matter and

Title Presenter name DATE Towards a More Sustainable World.

A B Combined consent and compound HIPAA authorization

Mixtures and Compounds

Lineage committment in mammary progenitor cells.

Simplifying Expressions

Peptide-membrane Interactions: Peptide Insertion

The Plasma Membrane.

Presentation transcript:

Tuning lipid mixtures to induce or suppress domain formation across leaflets of unsupported asymmetric bilayers Marcus D. Collins and Sarah L. Keller

Background Lipid bilayers are composed of two leaflets Lipids of the leaflets may separate into domains (grouping of lipid types) Domain formation is dependent on the types of lipids present Domain formation in one leaflet may induce or suppress domain formation in its partner leaflet.

Lipid Domains

Experiment Procedure Created 4 types of artificial leaflets: A, B, C and inner –Three different lipids were used –leaflets differ in lipid type percentages. Comp A: uniformComp C: domains Comp B: domainsInner: uniform Bilayers were created in every combination of leaflet type Induction or suppression of domain formation was determined.

Results B induces domains in A A suppresses domains in C B induces domains in Inner Inner suppresses domains in C

Conclusions and Further Research Different types of lipids have different affinities towards domain formation Test the effects of obstacles within the leaflet. Model lipid domain formation.