Persistence of marrow stromal cells implanted into acutely infarcted myocardium: Observations in a xenotransplant model Derek J. MacDonald, MD, Jun Luo,

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Persistence of marrow stromal cells implanted into acutely infarcted myocardium: Observations in a xenotransplant model Derek J. MacDonald, MD, Jun Luo, MD, Takayuki Saito, MD, PhD, Minh Duong, BSc, Pierre-Luc Bernier, MD, Ray C.J. Chiu, MD, PhD, Dominique Shum-Tim, MD The Journal of Thoracic and Cardiovascular Surgery Volume 130, Issue 4, Pages 1114-1121 (October 2005) DOI: 10.1016/j.jtcvs.2005.04.033 Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 1 Gross heart specimen and phenotypic changes of MSCs. Hearts were harvested at 4 weeks after cell injection (MSC group, I) or culture medium injection (control group, II). Hearts were then stained for b-gal activity. Intense blue discoloration was seen on infarct and peri-infarct areas of heart in MSC group (A). Hematoxylin and eosin staining is shown at low power (B, 40×) and eosin-only staining is shown at high power (C, 200×, and D, 400×) of the peri-infarct zones of myocardium. Note β-galactosidase–positive cells (arrows) in peri-infarcted myocardium (C and D) are more elongated and phenotypically resemble surrounding host cardiomyocytes. Immunostaining was done for cardiomyocyte-specific contractile protein troponin IC (arrow in E, 400×). In group III, histologic examination of LV cross-sections after injection of lacZ labeled mouse skin fibroblasts (arrow) showed extensive cellular infiltration within 1 day, particularly in subgroup IIIA with associated myocardial ischemic injury. However, in both subgroups with (IIIA) and without (IIIB) coronary ligations, only few remaining surviving labeled xenogeneic fibroblasts were found on day 4 after implantation (F, hematoxylin and eosin, 40×). The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1114-1121DOI: (10.1016/j.jtcvs.2005.04.033) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 2 Indices of LV performance. Echocardiographic data 4 weeks after MSC (group I, black bars) or culture medium (group II, white bars) injection. Asterisk indicates P = .007 for group I vs II; double asterisk indicates P = .029 for group I vs II. The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1114-1121DOI: (10.1016/j.jtcvs.2005.04.033) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

Figure 3 Components of stroke volume. Echocardiogram data performed at 4 weeks after MSC (group I, diamonds) or culture medium (group II, squares) injection comparing LVEDV and LVESV. Asterisk indicates P = .009 for group I vs II. The Journal of Thoracic and Cardiovascular Surgery 2005 130, 1114-1121DOI: (10.1016/j.jtcvs.2005.04.033) Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions