Developmental sperm contributions: fertilization and beyond

Slides:

Advertisements

Similar presentations

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Advertisements

Adverse effect of paroxetine on sperm

Ashok Agarwal, Ph. D. , H. C. L. D. , Rakesh Sharma, Ph. D

Ruben D. Motrich, M. Sc. , Andres A. Ponce, Ph. D. , Virginia E

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolas Garrido, Ph

Outdoor air pollution and sperm quality

Bjørn Bay, M. D. , Erik Lykke Mortensen, M. Sc

Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates Chun-Chia Huang, M.Sc., David.

Michael M. Alper, M.D. Fertility and Sterility

Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro fertilization success Luke Simon,

Denny Sakkas, Ph.D., Juan G. Alvarez, M.D., Ph.D.

Marcos Meseguer, Ph. D. , Rebeca Santiso, Ph. D. , Nicolás Garrido, Ph

Distribution of male infertility specialists in relation to the male population and assisted reproductive technology centers in the United States Ajay.

The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated.

Sperm head morphology is related to high deoxyribonucleic acid stainability assessed by sperm chromatin structure assay Armand Zini, M.D., Simon Phillips,

Gyun Jee Song, Ph.D., Vivian Lewis, M.D. Fertility and Sterility

Testosterone use in the male infertility population: prescribing patterns and effects on semen and hormonal parameters Mary K. Samplaski, M.D., Yasir.

Live birth of twins derived from zona-free oocytes

Ying-Hung Lin, Ph. D. , Chuan-Kai Chou, M. Sc. , Yu-Ching Hung, M. Sc

Ahmad Majzoub, M. D. , Nicholas N. Tadros, M. D. , A

Diagnostic accuracy of sperm chromatin dispersion test to evaluate sperm deoxyribonucleic acid damage in men with unexplained infertility Cinthia M.

Comparison of reproductive outcome in oligozoospermic men with high sperm DNA fragmentation undergoing intracytoplasmic sperm injection with ejaculated.

How old is too old? Challenges faced by clinicians concerning age cutoffs for patients undergoing in vitro fertilization Robert L. Klitzman, M.D. Fertility.

Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study Ashok Agarwal, Ph.D., H.C.L.D., Fnu Deepinder,

Ultrastructural findings in semen samples of infertile men infected with Chlamydia trachomatis and mycoplasmas Guadalupe Gallegos-Avila, M.D., Marta.

Akanksha Mehta, M. D. , Alexander Bolyakov, M. S. , Peter N

Oocyte cryopreservation

Ramadan Saleh, M. D. , Reda Z. Mahfouz, M. D. , Ashok Agarwal, Ph. D

A new real-time morphology classification for human spermatozoa: a link for fertilization and improved embryo quality Nino Guy Cassuto, M.D., Dominique.

Curcumin ameliorates aflatoxin-induced toxicity in mice spermatozoa

Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples Rosaria Schillaci, Ph.D., Giuseppina Capra,

Mieke Carine Wim Eeckhaut, Ph.D. Fertility and Sterility

Xiuye Xing, M. D. , Ph. D. , Han Zhao, M. D. , Ph. D. , Mei Li, M. Sc

Laura Kelly Thomson, B. Med. Sc. , Steven Denis Fleming, Ph. D

DNA fragmentation of normal spermatozoa negatively impacts embryo quality and intracytoplasmic sperm injection outcome Conrado Avendaño, M.S., Anahí.

Epidemiology of male and female reproductive disorders and impact on fertility regulation and population growth Kurt T. Barnhart, M.D., M.S.C.E. Fertility.

The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation? Gül Özdemirler Erata, Ph.D.,

Normal birth after single-embryo transfer in a patient with excessive polypronuclear zygote formation following in vitro fertilization and intracytoplasmic.

Inequity between male and female coverage in state infertility laws

Ongoing twin pregnancy after rescue intracytoplasmic sperm injection of unfertilized abnormal oocytes Navid Esfandiari, D.V.M., Ph.D., H.C.L.D., E. Anne.

Evaluation of diagnostic testis biopsy and the repetition of testicular sperm extraction surgeries in infertility patients Alayman Hussein, M.B.B.C.H.,

Fragmentation of DNA in morphologically normal human spermatozoa

Prospective, observational study of the depth of anesthesia during oocyte retrieval using a total intravenous anesthetic technique and the Bispectral.

Human papillomavirus found in sperm head of young adult males affects the progressive motility Carlo Foresta, M.D., Andrea Garolla, M.D., Daniela Zuccarello,

Ri-Cheng Chian, Ph.D., Peter S. Uzelac, M.D., Geeta Nargund, M.D.

In vitro fertilization and breast cancer: is there cause for concern?

Zsolt P. Nagy, M. D. , Ph. D. , Laura F. Rienzi, Ph. D. , Filippo M

Roberto Ensenat-Waser, Ph. D. , Antonio Pellicer, M. D. , Ph. D

Cameron Gilbert, M. Sc. , Maria Valois, M. D. , FRCPC, Ph. D

Impact of different patterns of sperm chromosomal abnormalities on the chromosomal constitution of preimplantation embryos Lorena Rodrigo, B.Sc., Vanessa.

Complex chromosome abnormality in patients with in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) S.P. Weng, T.C.J. Wu, S.P. Kang,

Pregnancy outcome in infertile patients with polycystic ovary syndrome who were treated with metformin Samuel S. Thatcher, M.D., Ph.D., Elizabeth M.

Natalia Rougier, M. D. , Heydy Uriondo, M. Sc. , Sergio Papier, M. D

L. Scott, J. Bernsten, K. DeLegge, J. Hill, N. Ramsing

Sperm selection by swim-up in terms of deoxyribonucleic acid fragmentation as measured by the sperm chromatin dispersion test is altered in heavy smokers

Peter J. Stahl, M. D. , Chava Cogan, B. S. , Akanksha Mehta, M. D

Counteraction during movement of spermatozoa by Trichomonas vaginalis observed by visual image analysis: a possible cause of female infertility Viroj.

Controlled ovarian hyperstimulation protocols for in vitro fertilization: two decades of experience after the birth of Elizabeth Carr Murat Arslan, M.D.,

Variability in testis biopsy interpretation: implications for male infertility care in the era of intracytoplasmic sperm injection Matthew R. Cooperberg,

David H. Barad, M.D., M.S., Norbert Gleicher, M.D.

Marcia E. Garcez, M. Sc. , Cátia dos Santos Branco, M. Sc

In vitro sildenafil citrate use as a sperm motility stimulant

Saad Elzanaty, M.D., Ph.D., Johan Malm, M.D., Ph.D.

Are sperm DNA fragmentation, hyperactivation, and hyaluronan-binding ability predictive for fertilization and embryo development in in vitro fertilization.

Brooks A. Keel, Ph.D., H.C.L.D.(A.B.B.) Fertility and Sterility

Tamer M. Said, M. D. , Geetha Ranga, M. D. , Ashok Agarwal, Ph. D. , H

Claire E. Beyer, B. Sc. , Bettina Kayler, B. M. Sc

Yinghui Ye, M. D. , Ph. D. , Chenming Xu, PhD. , Yuli Qian, B. Sc

Alinne Colin, M. D. , Gerardo Barroso, M. D. , M. Sc

Gülşen Aktan, Ph. D. , Semra Doğru-Abbasoğlu, M. D

Presentation transcript:

Developmental sperm contributions: fertilization and beyond Gerardo Barroso, M.D., M.Sc., Carlos Valdespin, M.D., Eva Vega, M.Sc., Ruben Kershenovich, M.D., Rosaura Avila, B.Sc., Conrado Avendaño, M.D., Sergio Oehninger, M.D., Ph.D. Fertility and Sterility Volume 92, Issue 3, Pages 835-848 (September 2009) DOI: 10.1016/j.fertnstert.2009.06.030 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Schematic of the journey of the sperm cell during spermatogenesis and transit through the male and female tracts (modified from reference 1, with permission). Fertility and Sterility 2009 92, 835-848DOI: (10.1016/j.fertnstert.2009.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Critical steps involved in sperm-oocyte interaction leading to pronuclear formation and syngamy. Fertility and Sterility 2009 92, 835-848DOI: (10.1016/j.fertnstert.2009.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Scheme of the ubiquitin-proteasome pathway in fertilization. Fertility and Sterility 2009 92, 835-848DOI: (10.1016/j.fertnstert.2009.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Three different patterns of DNA fragmentation (TUNEL assay) frequently observed in infertile men (left: granular appearance throughout the sperm head; center: predominance of vacuolated appearance; right: homogeneous fluorescence throughout nuclear area). Fertility and Sterility 2009 92, 835-848DOI: (10.1016/j.fertnstert.2009.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 5 Morphologically normal sperm with fragmented DNA (normal-SFD) in the same semen sample of patients currently undergoing ICSI. Simultaneous assessment of normal sperm morphology (phase contrast, unstained) and DNA fragmentation (TUNEL) after swim-up separation (left side: phase contrast; right side: TUNEL fluorescence). (A) Normal spermatozoon with DNA fragmentation, (B) abnormal spermatozoon (slightly abnormal form) with DNA fragmentation, (C) morphologically abnormal spermatozoa (severely amorphous) with DNA fragmentation, and (D) morphologically abnormal spermatozoa (tapered form) without DNA fragmentation. Fertility and Sterility 2009 92, 835-848DOI: (10.1016/j.fertnstert.2009.06.030) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions