Sébastien Colette, Ph. D. , Sylvie Defrère, Ph. D

Slides:



Advertisements
Similar presentations
First series of 18 pregnancies after ulipristal acetate treatment for uterine fibroids Mathieu Luyckx, M.D., Jean-Luc Squifflet, M.D., Ph.D., Pascale Jadoul,
Advertisements

TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation  Gabriela dos Santos Hidalgo, M.Sc.,
Sphingosine pathway deregulation in endometriotic tissues
Sachiko Matsuzaki, M. D. , Takashi Murakami, M. D. , Shigeki Uehara, M
The endometria of patients with endometriosis show higher expression of class I human leukocyte antigen than the endometria of healthy women  María del.
Anca Chelariu-Raicu, M. D. , Cornelia Wilke, Ph. D. , Melanie Brand, M
Surgical removal of endometriotic lesions alters local and systemic proinflammatory cytokines in endometriosis patients  Stephany P. Monsanto, M.Sc.,
Increased expression of c-fos protein associated with increased matrix metalloproteinase-9 protein expression in the endometrium of endometriotic patients 
Local injury to the endometrium in controlled ovarian hyperstimulation cycles improves implantation rates  Liang Zhou, M.D., Rong Li, M.D., Ph.D., Rong.
Effect of recombinant human TNF–binding protein-1 and GnRH antagonist on mRNA expression of inflammatory cytokines and adhesion and growth factors in.
Eutopic endometrial interleukin-18 system mRNA and protein expression at the level of endometrial-myometrial interface in adenomyosis patients  Hong-Yuan.
Human leukocyte antigen-G, a ligand for the natural killer receptor KIR2DL4, is expressed by eutopic endometrium only in the menstrual phase  Masaaki.
Reinaldo González-Ramos, M. D. , Ph. D. , Anne Van Langendonckt, Ph. D
Vitrification and xenografting of human ovarian tissue
Glycodelin A is expressed differentially in normal human endometrial tissue throughout the menstrual cycle as assessed by immunohistochemistry and in.
TAGLN expression is deregulated in endometriosis and may be involved in cell invasion, migration, and differentiation  Gabriela dos Santos Hidalgo, M.Sc.,
Growth rates of ovarian follicles during natural menstrual cycles, oral contraception cycles, and ovarian stimulation cycles  Angela R. Baerwald, Ph.D.,
Expression of motility-related molecule Cdc42 in endometrial tissue in women with adenomyosis and ovarian endometriomata  Gaia Goteri, M.D., Andrea Ciavattini,
Live birth after allografting of ovarian cortex between monozygotic twins with Turner syndrome (45,XO/46,XX mosaicism) and discordant ovarian function 
The expression of estrogen receptors as well as GREB1, c-MYC, and cyclin D1, estrogen-regulated genes implicated in proliferation, is increased in peritoneal.
Expression of microtubule associated protein 2 and synaptophysin in endometrium: high levels in deep infiltrating endometriosis lesions  Martina Gori,
Aldo Cavallini, B. Sc. , Leonardo Resta, M. D
Khaleque Newaz Khan, M. D. , Ph. D. , Michio Kitajima, M. D
Increased expression of p21-activated kinase in adenomyosis
Regine Gaetje, M. D. , Uwe Holtrich, Ph. D. , Thomas Karn, Ph. D
Michio Kitajima, M. D. , Ph. D. , Sylvie Defrère, Ph. D
Interleukin-18 system messenger RNA and protein expression in human endometrium during the menstrual cycle  Hong-Yuan Huang, M.D., She-Hung Chan, B.Sc.,
Vascular density and distribution of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) are significantly higher in patients with.
Induction of endometriotic nodules in an experimental baboon model mimicking human deep nodular lesions  Olivier Donnez, M.D., Anne Van Langendonckt,
Dioxin may promote inflammation-related development of endometriosis
Invasion process of induced deep nodular endometriosis in an experimental baboon model: similarities with collective cell migration?  Olivier Donnez,
Immunohistochemical detection of aquaporin expression in eutopic and ectopic endometria from women with endometriomas  Xiu-Xiu Jiang, M.D., Riu-Jin Wu,
Olivier Donnez, M. D. , Michelle Soares, M. D. , Sylvie Defrère, Ph. D
Lusine Aghajanova, M. D. , Ph. D. , Signe Altmäe, Ph. D
Xiao-Yu Pan, Ph. D. , Xue Li, M. D. , Zhan-Ping Weng, Ph. D
With the advent of selective progesterone receptor modulators, what is the place of myoma surgery in current practice?  Jacques Donnez, M.D., Ph.D., Olivier.
Evaluation of a human ovarian follicle isolation technique to obtain disease-free follicle suspensions before safely grafting to cancer patients  Michelle.
Both host and graft vessels contribute to revascularization of xenografted human ovarian tissue in a murine model  Anne-Sophie Van Eyck, M.D., Caroline.
Immunohistochemical detection of heparanase-1 expression in eutopic and ectopic endometrium from women with endometriosis  Xiulong Xu, Ph.D., Jianchi.
Up-regulation of endocrine gland-derived vascular endothelial growth factor but not vascular endothelial growth factor in human ectopic endometriotic.
Deoxyribonucleic acid methyltransferases and methyl-CpG-binding domain proteins in human endometrium and endometriosis  Kim J.A.F. van Kaam, M.D., Bert.
Protein profiling of human endometrial tissues in the midsecretory and proliferative phases of the menstrual cycle  Tanu Parmar, M.S., Sushama Gadkar-Sable,
Rahi Victory, M. D. , F. R. C. S. C. , Ghassan M. Saed, Ph. D
Vascular endothelial growth factor polymorphisms (−460C/T, +405G/C, and 936C/T) and endometriosis: their influence on vascular endothelial growth factor expression 
Murat Ulukus, M. D. , E. Cagnur Ulukus, M. D. , Ege N
Ching-wen Cheng, Ph. D. , Stephen K. Smith, M. D. , D
Macrophage migration inhibitory factor expression in the intrauterine endometrium of women with endometriosis varies with disease stage, infertility status,
Immunohistochemical localization of growth factors after cryopreservation and 3 weeks' xenotransplantation of human ovarian tissue  Anu David, M.Sc.,
Ana Luiza L. Rocha, M. D. , Patrizia Carrarelli, B. Sc
Introduction Fertility and Sterility
Mara Curaba, M. Sc. , Jonathan Poels, M. Sc
Changes in cytokine levels of patients with ovarian endometriosis after treatment with gonadotropin-releasing hormone analogue, ultrasound-guided drainage,
Fei Wang, M. D. , Ph. D. , Zeqing Wen, M. D. , Hui Li, Ph. D
CDB-2914, a novel selective progesterone receptor modulator, differentially regulates endometrial gene expression in the proliferative phase of the menstrual.
Iron storage is significantly increased in peritoneal macrophages of endometriosis patients and correlates with iron overload in peritoneal fluid  Jean-Christophe.
Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D
Anne Van Langendonckt, Ph. D. , Françoise Casanas-Roux, Ph. D
Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease  Idhaliz Flores, Ph.D., Elizabeth Rivera,
Circulating microRNAs as potential biomarkers for endometriosis
Elevated levels of gremlin-1 in eutopic endometrium and peripheral serum in patients with endometriosis  Guihua Sha, Ph.D., Yan Zhang, M.D., Chengyan.
Cleophas M. Kyama, Ph. D. , Attila Mihalyi, Ph. D
Jacques Donnez, M. D. , Ph. D. , Maria Mercedes Binda, Ph. D
Expression of vascular endothelial growth factor and thrombospondin-1 mRNA in patients with endometriosis  Xian-Jie Tan, Ph.D., Jing-He Lang, M.D., Dong-Yuan.
Jacques Donnez, M. D. , Ph. D. , Jean Squifflet, M. D
Quantitative expression of apoptosis-regulating genes in endometrium from women with and without endometriosis  Donald P. Braun, Ph.D., Jianchi Ding,
Nerve fibers and menstrual cycle in peritoneal endometriosis
A selective cyclooxygenase-2 inhibitor suppresses the growth of endometriosis with an antiangiogenic effect in a rat model  Daniel Escorsim Machado, M.Sc.,
Patrizia Carrarelli, M. Sc. , Ana Luiza Lunardi Rocha, M. D
Daniel R. Grow, M.D., Khosro Iromloo, M.D.  Fertility and Sterility 
What if deep endometriotic nodules and uterine adenomyosis were actually two forms of the same disease?  Jacques Donnez, M.D., Ph.D., Marie-Madeleine.
Presentation transcript:

Differential expression of steroidogenic enzymes according to endometriosis type  Sébastien Colette, Ph.D., Sylvie Defrère, Ph.D., Olivier Van Kerk, B.Sc., Anne Van Langendonckt, Ph.D., Marie-Madeleine Dolmans, M.D., Ph.D., Jacques Donnez, M.D., Ph.D.  Fertility and Sterility  Volume 100, Issue 6, Pages 1642-1649 (December 2013) DOI: 10.1016/j.fertnstert.2013.08.003 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Q-PCR results in eutopic endometrium from control patients and patients with endometriosis analyzed according to the menstrual cycle phase of patients. Box plots show mRNA expression differences between menstrual cycle phases (patients in the proliferative [Prolif] or secretory [Secret] phase or under oral contraception [OC]), for control (disease-free) endometrium (CE; proliferative, n = 7; secretory, n = 5; OC, n = 3) (A, C, E, G) and eutopic endometrium (EE) recovered from patients with endometriosis (proliferative, n = 8; secretory, n = 9; OC, n = 16) (B, D, F, H). A significant increase in HSD17B2 expression was detected during the secretory phase in CE but not in EE. Statistical analysis was performed using the Kruskal-Wallis test, followed by Dunn's post hoc test. *P<.05. Fertility and Sterility 2013 100, 1642-1649DOI: (10.1016/j.fertnstert.2013.08.003) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Q-PCR results in endometriotic lesions. Box plots represent q-PCR results in eutopic endometrium from patients with endometriosis (EE, n = 17), peritoneal lesions (PL, n = 9), ovarian lesions (OL, n = 7), and rectovaginal lesions (RL, n = 6) for HSD17B1 (A), HSD17B2 (B), STS (C), and EST (D) protein expression. HSD17B2 mRNA expression was lower in ovarian and rectovaginal lesions (B) than in their matched eutopic endometrium, while STS mRNA expression was higher in rectovaginal lesions (C) than in ovarian lesions. An increase in the HSD17B1/HSD17B2 ratio was observed in rectovaginal lesions (compared with their matched eutopic endometrium), and in ovarian lesions (compared with their matched eutopic endometrium and peritoneal lesions) (E). The ratio between STS and EST was always highly in favor of estrogens (F). Statistical analysis was performed using the Kruskal-Wallis test, followed by Dunn's post hoc test. *P<.05; **P<.01; ***P<.001. Fertility and Sterility 2013 100, 1642-1649DOI: (10.1016/j.fertnstert.2013.08.003) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Immunohistochemical photomicrographs of lesions and positive control tissues. Immunohistochemical results are shown in a series of representative pictures. Syncytiotrophoblasts were found to be positive for HSD17B1 and STS in control (placental) tissue (A–G), while endothelial cells expressed HSD17B2 (D). Glandular expression was observed for all three enzymes evaluated in eutopic endometrium and endometriotic tissues (B, C, E, F, H, I). Original magnification, ×100 (A, B, D, E, G, I), ×200 (C–F). Fertility and Sterility 2013 100, 1642-1649DOI: (10.1016/j.fertnstert.2013.08.003) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions