Chondrocyte-Specific Loss of the Branched Actin Mediator ARP2/3 Results in Growth Plate Fusion and Proteoglycan Loss in Articular Cartilage B.O. Diekman,

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Chondrocyte-Specific Loss of the Branched Actin Mediator ARP2/3 Results in Growth Plate Fusion and Proteoglycan Loss in Articular Cartilage B.O. Diekman, J.D. Rotty, V. Ulici, J.E. Bear, R.F. Loeser, N.E. Sharpless Osteoarthritis and Cartilage Volume 25, Pages S277-S278 (April 2017) DOI: 10.1016/j.joca.2017.02.469 Copyright © 2017 Terms and Conditions

Figure 1: H&E stained section of tibiae from 26 day old mice Figure 1: H&E stained section of tibiae from 26 day old mice. Homozygous Arp2/3 loss was driven by a Col2-Cre (right) and littermate control retaining one copy of Arp2/3 is shown (left). Boxes indicate approximate location of inset (bottom). All scale bars are 50 μm. Osteoarthritis and Cartilage 2017 25, S277-S278DOI: (10.1016/j.joca.2017.02.469) Copyright © 2017 Terms and Conditions

Figure 2: H&E stained sections of the tibia of mice at 6 months (top) and 12 months (bottom) of age. All mice had Aggrecan-CreERT2 and received tamoxifen at 4 months of age, but controls retained one copy of functional Arp2/3. Scale bars are 100 μm. Osteoarthritis and Cartilage 2017 25, S277-S278DOI: (10.1016/j.joca.2017.02.469) Copyright © 2017 Terms and Conditions

Figure 3. Safranin-O stained sections were graded for articular cartilage structure (A) and proteoglycan loss (B). A semiquantitative score of 0–12 was summed across four quadrants of the joint (high scores consistent with OA, max = 48). # indicates p<0.05 to all other groups. Osteoarthritis and Cartilage 2017 25, S277-S278DOI: (10.1016/j.joca.2017.02.469) Copyright © 2017 Terms and Conditions