Metastable Pluripotent States in NOD-Mouse-Derived ESCs

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Metastable Pluripotent States in NOD-Mouse-Derived ESCs

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Metastable Pluripotent States in NOD-Mouse-Derived ESCs Jacob Hanna, Styliani Markoulaki, Maisam Mitalipova, Albert W. Cheng, John P. Cassady, Judith Staerk, Bryce W. Carey, Christopher J. Lengner, Ruth Foreman, Jennifer Love, Qing Gao, Jongpil Kim, Rudolf Jaenisch Cell Stem Cell Volume 16, Issue 5, Pages 566-568 (May 2015) DOI: 10.1016/j.stem.2015.04.007 Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure S9 Characterization of Pluripotency Markers (A and B) Semi-quantitative RT-PCR analysis of transcripts from pluripotency genes and actin in the indicated pluripotent lines. Cell Stem Cell 2015 16, 566-568DOI: (10.1016/j.stem.2015.04.007) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure S10 Characterization of EpiSCs-iPSCs (A) Immuno-fluorescence stain of 129 iPSEpiSC (also referred to as Epi-iPS) cells for Oct4, Nanog, and SSEA1. (B) Southern blot analysis for detection of viral integration for lentiviruses encoding the reprogramming factors. Black arrows indicate endogenous bands, and empty arrows indicate integration bands. Klf4 was probed with an internal digest (EcoRI) which detects ∼1.2kb transgene band. (∗) indicates background bands preset in all samples. Cell Stem Cell 2015 16, 566-568DOI: (10.1016/j.stem.2015.04.007) Copyright © 2015 Elsevier Inc. Terms and Conditions