Strategies for annotation of a genome Approaches Updating GO annotations

Approaches for annotation of a genome Automated approaches 2. Manual approaches a. InterPro2GO a. Sequence similarity b. Experimental evidence

Annotations via sequence similarity Alignments published in literature Analysis using full length protein

Annotation from experimental evidence Focus on known genes Identify literature relevant to that gene a. using gene names, species name Complete annotation set for a gene Annotate all available experimental data “unknown” annotations ok

Updating GO annotations GO annotations will never be “done” Ongoing process New experimental data More specific annotation Replace obsolete terms