Current Technology for the Derivation of Pluripotent Stem Cell Lines from Human Embryos  Kouichi Hasegawa, Jordan E. Pomeroy, Martin F. Pera  Cell Stem Cell  Volume 6, Issue 6, Pages 521-531 (June 2010) DOI: 10.1016/j.stem.2010.05.010 Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 1 Schematic of the Process of Human Embryonic Stem Cell Line Establishment Cell Stem Cell 2010 6, 521-531DOI: (10.1016/j.stem.2010.05.010) Copyright © 2010 Elsevier Inc. Terms and Conditions

Figure 2 hESC Colonies Derived and Cultured under Various Conditions Full grown day 6 human blastocyst (A) and isolated ICM (B). (C) shows an attached ICM with growing pluripotent cells, 1 day after seeding on feeder layer. Morphologies of hESC colonies growing in KSR medium on conventional MEF feeder-layer (D), in KSR medium on human dermal fibroblast feeder-layer (E), in KSR medium on xeno-free prepared human dermal fibroblast feeder-layer (F), in xeno-and feeder-free medium mTesR2 (G). (A)–(C) are courtesy of Dr Suemori and were generated according to the methods published in Suemori et al. (2006). Cell Stem Cell 2010 6, 521-531DOI: (10.1016/j.stem.2010.05.010) Copyright © 2010 Elsevier Inc. Terms and Conditions