Cross-reactivity patterns of T cells specific for iodinated contrast media Marianne Lerch, MD, Monika Keller, PhD, Markus Britschgi, PhD, Gisele Kanny,

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Presentation transcript:

Cross-reactivity patterns of T cells specific for iodinated contrast media Marianne Lerch, MD, Monika Keller, PhD, Markus Britschgi, PhD, Gisele Kanny, MD, PhD, Valerie Tache, Daphne A. Schmid, PhD, Andreas Beeler, MSc, Basil O. Gerber, PhD, Michael Luethi, Andreas J. Bircher, MD, Cathrine Christiansen, MSc, Werner J. Pichler, MD Journal of Allergy and Clinical Immunology Volume 119, Issue 6, Pages 1529-1536 (June 2007) DOI: 10.1016/j.jaci.2007.02.007 Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Lymphocyte transformation test of patient 2. Proliferation of PBMCs from patient 2 measured by 3H-thymidine incorporation (counts per minute) after stimulation with different concentrations of CMs (0.1, 1, 5, 10 mgI/mL, respectively) is shown. The results are representative of 2 independent experiments. The error bars represent 1 SEM. conc., Concentration; w/o, without; PPD, tuberculin purified protein derivative. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Upregulation of the early activation marker CD69 on PBMCs of patient 2 after stimulation with different skin test–positive CMs. A, The percentage of CD4+ cells showing CD69 upregulation is depicted as NMFI. B, CD69 upregulation on CD8+ cells. Shown is 1 representative experiment of 3 performed. w/o, Without. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 CFSE intensity distribution analysis of PBMCs of patient 2 after stimulation with different CMs for 7 days. Similar results were obtained for CD4+(A) as well as for CD8+ T cells (B). Shown is 1 representative experiment of 2 performed. w/o, Without. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Cross-reactivity pattern of 1 iomeprol-specific (A) and 1 iodixanol-specific (B) TCL of patient 2 after 14 days and restimulation with phytohemagglutinin. The results are representative of 2 independent experiments. Note that some weakly stimulatory CMs (iobitridol, iotrolan) were negative in the iodixanol-stimulated TCL, but not in the iomeprol-stimulated TCL. The error bars represent 1 SEM. conc., Concentration; w/o, without. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Cross-reactivity patterns of selected iohexol-specific and iomeprol-specific TCCs. In patient 1, 2 different patterns were found. TCC Om7 represents 1 of 2 iohexol-specific TCCs with a narrow cross-reactivity pattern, whereas TCC Om27 represents a specific TCC with a broad cross-reactivity pattern (A). In patient 2, the CD4+ TCC 4∗1/76 showed a broad cross-reactivity pattern. In contrast with this, the CD8+ TCC 3∗0.3/28 reacted only with iomeprol (B). conc., Concentration; incorp., incorporation; w/o, without. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 6 Dose-response of iodixanol-specific TCLs (A and B). In C, iohexol and its dimer iodixanol are schematically represented: the TCR might recognize the iodixanol as shown in a, b, or c. Because equimolar concentrations stimulated the TCLs very similarly, the dimeric structure does not seem to be important for TCR recognition (no recognition as shown in c). w/o, Without. Journal of Allergy and Clinical Immunology 2007 119, 1529-1536DOI: (10.1016/j.jaci.2007.02.007) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions