Rewiring Cas9 to Target New PAM Sequences

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Rewiring Cas9 to Target New PAM Sequences Virginijus Siksnys, Giedrius Gasiunas Molecular Cell Volume 61, Issue 6, Pages 793-794 (March 2016) DOI: 10.1016/j.molcel.2016.03.002 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Structural Mechanism of a Protospacer Adjacent Motif Sequence Recognition by SpCas9 The DNA target site for SpCas9 is composite and consists of two nucleotide sequences termed a protospacer and protospacer adjacent motif (PAM), respectively. Protospacer interaction motif (PIM) recognizes the 5′-GG-3′ PAM in the WT SpCas9. Mutations in PIM sequence enable recognition of altered PAM by an induced fit mechanism. Molecular Cell 2016 61, 793-794DOI: (10.1016/j.molcel.2016.03.002) Copyright © 2016 Elsevier Inc. Terms and Conditions