T3 increased J7-TRα1 cell migration and proliferation both in vitro and in vivo. T3 increased J7-TRα1 cell migration and proliferation both in vitro and in vivo. PAI-1 expression was determined in the (A) J7-TRα1 cells treated with or without 1 or 10 nm T3 for 24h using Western blotting. In vitro (B) migration and (C) proliferation were analyzed through a Transwell assay or MTT assay in J7-TRα1 cells in the absence or presence of 1 or 10 nm T3, as described under “Experimental Procedures.” D, The nude mice injected subcutaneously with J7-TRα1 cells that were divided into hyperthyroid, euthyroid and hypothyroid groups (n = 6 per group). The tumor sizes and weights of three groups were measured up to 25 days after inoculation of tumor cells. E, The metastasis index (fold, density of tumor numbers in per cm2 area) in the lung of J7-TRα1 cells (hyperthyroid, euthyroid and hypothyroid) are shown. The (F) tumor foci and PAI-1 expression were displayed by H&E staining and IHC, respectively. The tumor foci cover almost all areas seen in (F). Values are shown as fold induction relative to 0 nm T3 or hypothyroid. Differences were analyzed using the One-way ANOVA analysis, **p < 0.01; *p < 0.05. Cheng-Yi Chen et al. Mol Cell Proteomics 2012;11:M111.011270 © 2012 by The American Society for Biochemistry and Molecular Biology, Inc.