Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms Adam J. Doyle, MD, Eileen M. Redmond,

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Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms Adam J. Doyle, MD, Eileen M. Redmond, PhD, David L. Gillespie, MD, Peter A. Knight, MD, John P. Cullen, PhD, Paul A. Cahill, PhD, David J. Morrow, PhD Journal of Vascular Surgery Volume 62, Issue 2, Pages 464-470 (August 2015) DOI: 10.1016/j.jvs.2014.02.053 Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 1 Hedgehog (Hh)/vascular smooth muscle cell (vSMC) differentiation gene expression in nonaneurysmal and aneurysmal tissue from patients undergoing open repair of infrarenal abdominal aortic aneurysm (AAA). A and B, Representative Western blot (A) and cumulative protein data (B) for Sonic Hedgehog (SHh), α-actin, and SMC22α expression levels in nonaneurysmal and aneurysmal tissue samples of patients. C, Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of SHh and Ptc-1 messenger RNA (mRNA) levels in nonaneurysmal and aneurysmal tissue samples of patients. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 8. Journal of Vascular Surgery 2015 62, 464-470DOI: (10.1016/j.jvs.2014.02.053) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 2 Hedgehog (Hh)/Notch signaling component expression in nonaneurysmal and aneurysmal tissue from patients undergoing open repair of infrarenal abdominal aortic aneurysm (AAA). A and B, Representative Western blot (A) and cumulative protein data (B) for Notch receptor 1 intracellular domain (Notch 1 IC) and Hrt-1 and Hrt-2 expression levels in nonaneurysmal and aneurysmal tissue samples of patients. C, Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of Sonic Hedgehog (SHh), Notch ligand Delta like 4 (Dll4), Notch 1, and Hrt-1 and Hrt-2 messenger RNA (mRNA) levels in nonaneurysmal and aneurysmal tissue samples of patients. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 8. Journal of Vascular Surgery 2015 62, 464-470DOI: (10.1016/j.jvs.2014.02.053) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 3 Transforming growth factor β1 (TGF-β1) expression in nonaneurysmal and aneurysmal tissue segments from patients undergoing open repair of infrarenal abdominal aortic aneurysm (AAA). A, Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of TGF-β1 messenger RNA (mRNA) levels in nonaneurysmal and aneurysmal tissue samples of patients. B, Representative Western blot and cumulative protein data for TGF-β expression levels in nonaneurysmal and aneurysmal tissue samples of patients. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 8. Journal of Vascular Surgery 2015 62, 464-470DOI: (10.1016/j.jvs.2014.02.053) Copyright © 2015 Society for Vascular Surgery Terms and Conditions

Fig 4 The effect of Hedgehog (Hh) signaling inhibition on Notch/transforming growth factor β (TGF-β)/α-actin messenger RNA (mRNA) expression in human aortic smooth muscle cells (HASMCs). Quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA levels of Ptc-1, Gli-2, Notch 1, Hrt-2, α-actin, and TGF-β1 in HASMCs treated with cyclopamine (40 μM) for 24 hours. Data are normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and represent the mean ± standard error of the mean; n = 4. Journal of Vascular Surgery 2015 62, 464-470DOI: (10.1016/j.jvs.2014.02.053) Copyright © 2015 Society for Vascular Surgery Terms and Conditions