Presentation is loading. Please wait.

Presentation is loading. Please wait.

Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer.

Published byDaniela Bailey Modified over 5 years ago

Similar presentations

Presentation on theme: "Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer."— Presentation transcript:

1 Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer cells  Jessica A. Yu, MD, Howard Li, MD, Xianzhong Meng, MD, PhD, David A. Fullerton, MD, Raphael A. Nemenoff, PhD, John D. Mitchell, MD, Michael J. Weyant, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 144, Issue 6, Pages (December 2012) DOI: /j.jtcvs Copyright © Terms and Conditions

2 Figure 1 K-ras mutant lung cancer cell lines analyzed for baseline group IIa secretory phospholipase A2 (sPLA2IIa) expression. Expression varies with SW1573 and H2009 cells expressing more sPLA2IIa compared with H358 and A549 cells. Messenger RNA (mRNA) was evaluated by quantitative reverse-transcription polymerase chain reaction (RT-PCR), mean ± SEM, *P < .05, n = 5, run in triplicate (A). sPLA2IIa protein expression was evaluated by Western blot. Graph of densitometry, mean ± SEM, **P < .005, n = 4 (B). Immunofluorescence for sPLA2IIa was used for additional protein, n = 3 run in duplicate, sPLA2IIa = red, cell membrane = green, nuclei = blue (C). IgG, Immunoglobulin G; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

3 Figure 2 Group IIa secretory phospholipase A2 (sPLA2IIa) expression inhibition promotes concentration-dependent apoptosis and cell death in lung cancer cell lines, and cytotoxicity of sPLA2IIa inhibition parallels the level of sPLA2IIa expression. SW1573 and H2009 cells experience more apoptosis at 20 and 40 μmol/L compared with H358 and A549 cells. Percent of cells with annexin V staining were normalized to staining of the respective vehicle control, mean ± SEM, *P < .03 compared with SW1573, H2009. **P < .01 compared with vehicle control, n = 3-4 (A). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assay cell viability after sPLA2IIa inhibition. LD50 at 24 hours increases as sPLA2IIa expression decreases, mean ± SEM, *P < .003, n = 3 run in triplicate (B). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

4 Figure 3 Group IIa secretory phospholipase A2 (sPLA2IIa) inhibition attenuates nuclear factor kappa-b (NF-κB) activity in all cell lines, with a variable effect on extracellular signal-regulated kinase (ERK) 1/2 activity. NF-κB attenuation does not correlate with sPLA2IIa expression level. Both H2009 and H358 (white bar graphs) are mutant p53 and demonstrate a more modest decrease in NF-κB activity (vc, vehicle control). Representative Western blots with corresponding densitometry, mean ± SEM, *P < .05, **P < .01, #P < .001, n = 3-5. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

5 Figure 4 Human lung tumors express group IIa secretory phospholipase A2 (sPLA2IIa). Primary lung tumors (n = 15), invasive esophageal adenocarcinoma (n = 1), lung metastasis from colon adenocarcinoma primary (n = 1). K-ras mutant adenocarcinomas (gray) express increasing levels of sPLA2IIa with increasing stage. Squamous cell carcinomas (black) express higher levels of sPLA2IIa. Samples that did not include K-ras, ALK, or epidermal growth factor receptor (EGFR) mutant status indicate patients that did not consent to mutation analysis. Messenger RNA evaluated by quantitative reverse-transcription polymerase chain reaction (RT-PCR), samples run in triplicate. LCNEC, Large cell neuroendocrine carcinoma; mut, mutant; wt, wild-type; s/p, status post; XRT, radiation therapy. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © Terms and Conditions

Download ppt "Group IIa secretory phospholipase expression correlates with group IIa secretory phospholipase inhibition–mediated cell death in K-ras mutant lung cancer."

Similar presentations

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation 

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation 
Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome  Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome  Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.
Overexpression of CRM1: A Characteristic Feature in a Transformed Phenotype of Lung Carcinogenesis and a Molecular Target for Lung Cancer Adjuvant Therapy 

Overexpression of CRM1: A Characteristic Feature in a Transformed Phenotype of Lung Carcinogenesis and a Molecular Target for Lung Cancer Adjuvant Therapy 
Prognostic impacts of EGFR mutation status and subtype in patients with surgically resected lung adenocarcinoma  Kazuya Takamochi, MD, Shiaki Oh, MD,

Prognostic impacts of EGFR mutation status and subtype in patients with surgically resected lung adenocarcinoma  Kazuya Takamochi, MD, Shiaki Oh, MD,
Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling  Baoping Cao, MD, PhD, Weili Yang,

Silencing NKD2 by Promoter Region Hypermethylation Promotes Esophageal Cancer Progression by Activating Wnt Signaling  Baoping Cao, MD, PhD, Weili Yang,
Inhibition of hypoxia-induced cyclooxygenase-2 by Korean Red Ginseng is dependent on peroxisome proliferator-activated receptor gamma  Heewon Song, Young.

Inhibition of hypoxia-induced cyclooxygenase-2 by Korean Red Ginseng is dependent on peroxisome proliferator-activated receptor gamma  Heewon Song, Young.
Modulation of growth in human esophageal adenocarcinoma cells by group IIa secretory phospholipase A2  David Mauchley, MD, Xianzhong Meng, MD, PhD, Thomas.

Modulation of growth in human esophageal adenocarcinoma cells by group IIa secretory phospholipase A2  David Mauchley, MD, Xianzhong Meng, MD, PhD, Thomas.
Heat shock protein 27: Induction by gastroduodenal reflux in vivo and augmentation of human esophageal mucosal cell growth in vitro  David Mauchley, MD,

Heat shock protein 27: Induction by gastroduodenal reflux in vivo and augmentation of human esophageal mucosal cell growth in vitro  David Mauchley, MD,
MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes  S.J. Park, E.J. Cheon,

MicroRNA-558 regulates the expression of cyclooxygenase-2 and IL-1β-induced catabolic effects in human articular chondrocytes  S.J. Park, E.J. Cheon,
Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription.

Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription.
EGFR-GRB2 Protein Colocalization Is a Prognostic Factor Unrelated to Overall EGFR Expression or EGFR Mutation in Lung Adenocarcinoma  Maria I. Toki, MD,

EGFR-GRB2 Protein Colocalization Is a Prognostic Factor Unrelated to Overall EGFR Expression or EGFR Mutation in Lung Adenocarcinoma  Maria I. Toki, MD,
High-mobility group box-1 protein induces osteogenic phenotype changes in aortic valve interstitial cells  Bo Wang, PhD, Fei Li, MD, Chao Zhang, PhD,

High-mobility group box-1 protein induces osteogenic phenotype changes in aortic valve interstitial cells  Bo Wang, PhD, Fei Li, MD, Chao Zhang, PhD,
Upregulation of PD-L1 by EGFR Activation Mediates the Immune Escape in EGFR- Driven NSCLC: Implication for Optional Immune Targeted Therapy for NSCLC Patients.

Upregulation of PD-L1 by EGFR Activation Mediates the Immune Escape in EGFR- Driven NSCLC: Implication for Optional Immune Targeted Therapy for NSCLC Patients.
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
WT1 Promotes Invasion of NSCLC via Suppression of CDH1

WT1 Promotes Invasion of NSCLC via Suppression of CDH1
Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,

Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,
Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.
Knockdown of secretory phospholipase A2 IIa reduces lung cancer growth in vitro and in vivo  Jessica A. Yu, MD, David Mauchley, MD, Howard Li, MD, Xianzhong.

Knockdown of secretory phospholipase A2 IIa reduces lung cancer growth in vitro and in vivo  Jessica A. Yu, MD, David Mauchley, MD, Howard Li, MD, Xianzhong.
Rafal Dziadziuszko, MD, PhD, Anh T

Rafal Dziadziuszko, MD, PhD, Anh T
Combined treatment with cisplatin and sirolimus to enhance cell death in human mesothelioma  Mor-Li Hartman, PhD, John Matthew Esposito, BA, Beow Yong.

Combined treatment with cisplatin and sirolimus to enhance cell death in human mesothelioma  Mor-Li Hartman, PhD, John Matthew Esposito, BA, Beow Yong.

Similar presentations

Ads by Google