Microscope Use

Scale

Simple Microscope Light passes through only 1 lens. Example: magnifying glass

Compound Microscope Lets light pass through an object and then through two or more lenses.

Parts of the Microscope Drag the box to view the correct answer underneath. Ocular lens (Eyepiece) Parts of the Microscope Body Tube Nosepiece Arm Low Power Objective High Power Objective Stage Stage Clips Coarse Adjustment Diaphragm Fine Adjustment Light Always carry a microscope with one hand holding the arm and one hand under the base. Base

Microscope Parts & Their Functions Drag the box to view the correct answer underneath. Controls the amount of light entering the microscope diaphragm Makes large changes in focus Coarse adjustment Makes small changes in focus Fine adjustment Holds the slide in position Stage clips Has the greatest magnification High power objective Has the least magnification Low power objective

Fill in the table on your worksheet. What’s my power? To calculate the power of magnification, multiply the power of the ocular lens (eyepiece) by the power of the objective lens. What are the powers of magnification for each of the objectives we have on our microscopes? Fill in the table on your worksheet.

Microscope Care Always carry with 2 hands (arm and base) Only use lens paper for cleaning Do not force knobs Always store covered Keep objects clear of desk and cords

Using the Microscope Lower stage all the way Rotate nosepiece and click the low-power objective lens(shortest) into position over the stage Place the slide on the microscope Look into the eyepiece Use the Coarse Adjustment knob to locate the object Use the Fine Adjustment to sharpen the image

How to make a wet-mount slide … 1 – Get a clean slide and coverslip from your teacher. 2 - Place the object you are viewing in the center of the slide 3 – Place ONE drop of water on the object. Don’t use too much or the water will run off the edge and make a mess! 4 – Place the edge of the cover slip on one side of the water drop. 5 - Slowly lower the cover slip on top of the drop. Cover Slip Lower slowly 6 – Tap out the air bubbles. 7- Use a paper towel to blot up any excess water.

Stereoscopic Microscope Gives a three dimensional view of an object. (Examples: insects and leaves) Used for dissections

Electron microscopes – use a beam of electrons instead of a beam of light to magnify the image

Electron Microscopes can achieve 3D images using electrons

The Scanning Electron Microscope produces a 3-dimensional image of specimen’s surface features spider head of a butterfly

Scanning electron microscopy (SEM) Types of specimens: -Whole organisms -Natural tissue surfaces -Exposed tissue structure A flea magnified 50 000 X What is this?

Transmission electron microscopy (TEM). Allows the observation of molecules within cells Allows the magnification of objects in the order of 100, 000’s.

Transmission electron microscope (TEM) Provides for detailed study of the internal ultrastructure of cells a beam of electrons is transmitted through the specimen for a 2D view Longitudinal section of cilium Cross section of cilium 1 µm Figure 6.4 (b) cilia on rabbit lungs

Transmission electron microscope Chloroplast from a tobacco leaf H1N1 virus