Genetics & DNA Genetics Central Concepts: Genes allow for the storage and transmission of genetic information. They are a set of instructions encoded in the nucleotide sequence of each organism. Genes code for the specific sequences of amino acids that comprise the proteins characteristic to that organism.

ACTIVATOR: Complete the using the glossary in your book. Section 10 ACTIVATOR: Complete the using the glossary in your book. Section 10.2 Mendelian Genetics WORD DEFINITION Genetics _________________________________________________________________________________________________________________________________________________________________________________ Allele Dominant Recessive

ACTIVATOR: Complete the Vocab on page 277. Section 10 ACTIVATOR: Complete the Vocab on page 277. Section 10.2 Mendelian Genetics WORD DEFINITION Homozygous _________________________________________________________________________________________________________________________________________________________________________________ Heterozygous Genotype Phenotype

ACTIVATOR: Complete the Vocab on page 277. Section 10 ACTIVATOR: Complete the Vocab on page 277. Section 10.2 Mendelian Genetics WORD DEFINITION Law of Segregation _________________________________________________________________________________________________________________________________________________________________________________ Hybrid Law of Independent Assortment

GENETICS is the branch of biology that deals with inheritance.

Do you see any characteristics that these children share with their parents or their brothers and sisters?

DNA (Deoxyribonucleic Acid)

Genetic material of cells… GENES – units of genetic material that CODES FOR A SPECIFIC TRAIT Called NUCLEIC ACIDS DNA is made up of repeating molecules called NUCLEOTIDES

DNA is the genetic material that is passed from generation to generation. Cells -> Nucleus –> Chromosomes –> Gene –> Trait BIGGEST--------------------------------- smallest

A HISTORY OF DNA Discovery of the DNA double helix A. Frederick Griffith – Discovers that a factor in diseased bacteria can transform harmless bacteria into deadly bacteria (1928) B. Rosalind Franklin - X-ray photo of DNA. (1952) C. Watson and Crick - described the DNA molecule from Franklin’s X-ray. (1953)

Watson & Crick proposed… DNA had specific pairing between the nitrogen bases: ADENINE – THYMINE CYTOSINE - GUANINE DNA was made of 2 long stands of nucleotides arranged in a specific way called the “Complementary Rule”

What is DNA? Although the environment influences how an organism develops, the genetic information that is held in the molecules of DNA ultimately determines an organism’s traits. DNA achieves its control by determining the structure of proteins. Within the structure of DNA is the information for life—the complete instructions for manufacturing all the proteins for an organism.

Watson and Crick also proposed that DNA is shaped like a long zipper that is twisted into a coil like a spring. Each nucleotide has three parts: sugar, phosphate, nitrogenous base

DNA is shped in a Double Helix 1 2 3 4 5 P O 1 2 3 4 5 G C T A

adenine (A), cytosine (C), guanine (G), and thymine (T). There are four different nitrogenous bases found in DNA: adenine (A), cytosine (C), guanine (G), and thymine (T).

Chargaff’s Rule Adenine must pair with Thymine Guanine must pair with Cytosine Their amounts in a given DNA molecule will be about the same. T A G C

Air goes in Tires Gas goes in Cars DNA -> DNA (A) pairs with (T), The four nitrogenous bases of DNA nucleotides bond together in only one way: DNA -> DNA (A) pairs with (T), (C) pairs with (G). Air goes in Tires Gas goes in Cars

Genetic Diversity… Different arrangements of NUCLEOTIDES in a nucleic acid (DNA) provides the key to DIVERSITY among living organisms.

The Code of Life… A T C G T A T G C G G… The “code” of the chromosome is the SPECIFIC ORDER that bases occur. A T C G T A T G C G G…

Hydrogen bonds hold the two strands together between the bases.

2. When does replication occur? 3. Describe how replication works. Replication Quiz 1. What is DNA? 2. When does replication occur? 3. Describe how replication works. Use the complementary rule to create the complementary strand: A---___ G---__ C--___ T---__ A---__ C---__

DNA REPLICATION – When DNA copies itself DNA can be used as the hereditary material because it can make identical copies of itself.

THE #1 Thing to Remember is the DNA MAKES PROTEINS! DNA Replication

The DNA controls cellular activities by providing instructions for PROTEIN SYNTHESIS. It happens in 2 Steps

REMEMBER: when you transcribe DNA code to RNA code, DNA ->mRNA A -> U T -> A C <-> G REMEMBER: when you transcribe DNA code to RNA code, use U in place of T.

carries the DNA code to the ribosome for MESSENGER RNA (mRNA) carries the DNA code to the ribosome for protein synthesis. mRNA is formed by copying DNA as a pattern or TEMPLATE in a process called TRANSCRIPTION.

PRACTICE TRANSCRIPTION If the DNA code reads : A G C T G T A C A Then the mRNA strand would read : U C G A C A U G U REMEMBER: when you transcribe DNA code to RNA code, use U in place of T.

mRNA molecules are read three bases at a time. Each three base sequence is called a CODON.

carries amino acids to the ribosomes for protein synthesis TRANSFER RNA (tRNA) carries amino acids to the ribosomes for protein synthesis Protein Synthesis Click here to see it in action This process is called TRANSLATION.

DNA Transcribed RNA Translated Proteins

Each CODON represents one of the 20 amino acids, or START or STOP.

PROTEIN SYNTHESIS requires two steps: In SUMMARY: PROTEIN SYNTHESIS requires two steps: TRANSCRIPTION (DNA mRNA) and TRANSLATION (mRNA polypeptide) Polypeptide is a chain of amino acids that when folded up make a Protein

What polypeptide would be formed from this DNA strand?? TACTTCAAAATC AUGAAGUUUUAG met-lys-phe

A single mutation of the hemoglobin gene causes sickle cell anemia. Any change in the sequence of nucleotides in a DNA molecule is a GENE MUTATION. The mutation may result in changes that affect the structure and function of the protein made by that gene. A single mutation of the hemoglobin gene causes sickle cell anemia.

Misshapen sickle cells Sickle cell anemia Hemoglobin protein in red blood cells strikes 1 out of 400 African Americans limits activity, painful & may die young Normal round cells Misshapen sickle cells Only 1 out of 146 amino acids

A single base change in the DNA sequence. POINT MUTATION A single base change in the DNA sequence. The effect of the mutation varies from no effect at all to a mutation that can cause a serious disease. A mutation that affects the third position of the mRNA codon has the least effect on the production of a particular protein.

Does this change the sentence? Point Mutations One base change can change the meaning of the whole protein THEFATCATANDTHEREDRATRAN Does this change the sentence? A LITTLE! THEFATCARANDTHEREDRATRAN OR THEFATCATENDTHEREDRATRAN

A single base is either added to or deleted from the DNA sequence. FRAME SHIFT MUTATION A single base is either added to or deleted from the DNA sequence. The mutation affects every codon after the location of the mutation. Very serious effects. Causes a completely different amino acid chain to be produced.

Although mutations occur spontaneously, the rate of mutation can be increased by exposure to MUTAGENIC AGENTS. X rays, ultraviolet rays, radioactive substances, and cosmic rays are mutagenic agents. Mutagenic chemicals include formaldehyde, benzene, and asbestos fibers.

Mutations may be transmitted to the next generation ONLY if they are present in the gametes. Mutations in body cells may be passed on to new cells of the individual as a result of mitosis, but will NOT be transmitted to the offspring.

GEL ELECTROPHORESIS – “DNA fingerprinting” Uses RESTRICTION ENZYMES to cut DNA into pieces Then SORTS OUT the DNA by SIZE. You can COMPARE the banding patterns produced by the DNA piles to determine the relationships between specimens.