Molding PDMS Channels and an Embedded Detector Chamber

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Presentation transcript:

Molding PDMS Channels and an Embedded Detector Chamber Takaya Ueda MASc Candidate Simon Fraser University July 25, 2006

Overview Background Objective Motivation Design Results Conclusion Current work 16/11/2018

Background Microfluidics Delivering liquid through narrow channels (~µm diameter) Small sample and chemical volume Biomedical applications e.g. Single cell testing and analysis 16/11/2018

Background SU-8 Poly-dimethylsiloxane (PDMS) Negative photoresist Solid after cross-linking Poly-dimethylsiloxane (PDMS) Silicone elastomer Applied as mold Optically transparent once cured 16/11/2018

SU-8 and PDMS Using SU-8 and PDMS together can rapidly fabricate multiple copies of a structure: Pattern structures with SU-8 Pour PDMS on top of the SU-8 Peal off the PDMS after cure Repeat 1-3 as necessary Rapid processing enables fabrication of low cost disposable devices 16/11/2018

Motivation Cells react to laser light or drugs Reaction causes fluorescence Photo-multiplier tube (PMT) used to detect and analyze the light Problem: PMT and laser are large and expensive compared to the channels 16/11/2018

Final Objective Fabricate a single cell analysis system for fluorescence detection With integrated Photo-diode to replace the PMT LED to replace the laser 16/11/2018

Current Objective Fabricate 50 µm diameter channels Photo-detector mount Both fabricated by molding using SU-8 and PDMS 16/11/2018

Design Overview PDMS Channel Layer PDMS Detector Mount Photo-detector 16/11/2018

Channel Design Outlet Inlet Cell Retention Chamber 2800 µm Inlet for cell retention 50 µm 16/11/2018

Results Successful fabrication of SU-8 channel and detector mounts Problems with air bubbles in PDMS Air bubbles affect transparency of PDMS to the eye, photo-diode, and LED SU-8 Channels SU-8 Detector Mount PDMS Channel 16/11/2018

Conclusion Successful fabrication of the SU-8 structures PDMS process must be improved to eliminate air bubbles to yield usable devices 16/11/2018

Current Work PDMS results have improved because of better processing Currently researching world-to-chip electrical connections with PDMS 16/11/2018

Questions? 16/11/2018