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Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi.

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Presentation on theme: "Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi."— Presentation transcript:

1 Microbubble Generation for Medical Applications Shirley Zhang Faculty Mentor: Abraham Lee Graduate Student: Kanaka Hettiarachchi

2 Overview  Microfluidic devices for generating bubbles – test for optimal conditions  Create double layer bubbles to contain lipid and oil surrounding gas  Test stability and attachment of bubbles in vitro

3 Making Devices U.V. Silicon wafer Photoresist Mask PDMS Microfluidic channels Develop

4 Monolayer bubbles  Components: Lipid (DSPE/DPPE- PEG2000) Gas (PFC or N 2 )  Diameter: ~15 um ColorCam 10x magnification

5 Fluorescence  DiI mixed into lipids ColorCam 40x magnification

6 Double Layer Bubbles  Components: Lipid Oil Gas (PFC or N 2 )  No lipid shell – no concentrated fluorescence around bubbles FastCam

7 Problems DSPC/DSPE-PEG2K Triacetin N2N2 FastCam

8 Future Direction  Successfully make stable double layer bubbles  Microbubbles as a drug delivery system in cell culture

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