Zika Virus Pathogenic Viral Infection First observed in Zika Forest of Uganda in 1947 Infection in adult is mostly asymptomatic (1 in 5) headache, macular/maculopapular pruritic rash, arthralgias, and conjunctivitis. Sever congenital microcephaly of newborn Caused by infection by the Zika Flavivirus Typically transmitted by mosquito, primarily Aedes aegypti Blood transfusion, sexual transmission, perinatal transmission No Treatments or Vaccine (under development)

Team Workshop 1: List the experimental steps and reagents used to obtain the data above and the conclusions drawn?

FLAVIVIRUS Yellow Fever Dengue Fever Japanese encephalitis West Nile viruses Zika virus

Can you predict regions potentially important for receptor binding? Team Workshop 1: Use online tools to obtain the protein sequence for Zika and Dengue Envelope E protein. Calculate a protein sequence similarity alignment between Zika Virus E protein and Dengue Virus E protein. Can you predict regions potentially important for receptor binding? Can you provide support for your prediction? 49 138 306 390 Tim and Tam phosphotidyl serine receptors.

Team Workshop 3: Drug Discovery Use the RCSB to obtain the PDB file of zika virus View the Structure in Chimera and Select the E glycoprotein subunit only. Save as a new PDB file. Make sure it only contains the atomic coordinates for the E glycoprotein and only protein atoms. Open this file in Chimera and perform DockPrep and when finished save as a new pdb file. Download the MOl2 file for ivermectin (IVM) http://dosequis.colorado.edu/Courses/BrainWeb/Docs/IVM.mol2 Run SwissDock (one person from Group) with the Zika glycoprotein and ivermectin. 4ONR

How to find it there

Team Workshop 4: Use STICH to investigate Ivermectin MOA. 4ONR

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