Protein and enzymes c: Enzyme specificity

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Protein and enzymes c: Enzyme specificity National 5 Biology Protein and enzymes c: Enzyme specificity

Recap

What are we learning today? We are learning to: Give examples of enzyme controlled reactions to illustrate enzyme specificity Success criteria: An enzyme is specific to one substrate Give examples

What does enzyme specificity mean? The substance an enzyme acts on is the SUBSTRATE. What is made in the reaction is called the PRODUCT.

Experiment 2 Aim: to find out what is meant by enzyme specificity using enzymes and various substrates A Amylase and starch test for sugar using clinistix B Amylase and hydrogen peroxide test for oxygen using glowing splint C Catalase and hydrogen peroxide test for oxygen using glowing splint D Catalase and starch test for sugar using clinistix  

Experiment conclusions Catalase only acts on hydrogen peroxide Amylase only acts on starch Enzymes are SPECIFIC This means that they only work on one substrate Enzymes are complimentary in shape to their substrate

Enzymes are Specific KEYS are SPECIFIC to LOCKS ENZYMES are SPECIFIC to SUBSTRATES hydrogen peroxide catalase water oxygen

Enzymes are Specific Catalase (enzyme) will only act on hydrogen peroxide (substrate) catalase hydrogen peroxide water oxygen catalase Starch No reaction No products formed

Enzymes are Specific Only catalase will catalyse the breakdown of hydrogen peroxide catalase hydrogen peroxide water oxygen hydrogen peroxide No reaction No products formed amylase

Enzymes are Specific Remember: Enzymes are specific because of their shape. catalase hydrogen peroxide water oxygen hydrogen peroxide No reaction No products formed amylase The shape of amylase is different to catalase so it will not catalyse the reaction.

The Active Site Where a substrate attaches to an enzyme is called the active site The active site is where the reaction occurs The active site is complimentary to the shape of the substrate The amino acid sequence determines the shape of the active site

bbcbitesize Standard Grade Biology

Summary Exit ticket Draw a diagram (not like one you have seen today) to show what you understand about enzyme specificity

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