A Bandwidth Detection System to Monitor Single Cells

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Presentation transcript:

A Bandwidth Detection System to Monitor Single Cells Jerry Wilmink and Jonathon Wells Advisor: Franz Baudenbacher BME 273, Group 9

Presentation Overview DARPA and BioMEMS Our Project Design Overview of Project Progress Future Plans

DARPA at Vanderbilt BioMEMS Device Funded by DARPA Cell based sensing technology Monitor Cell Metabolism Subnanoliter volumes Bio MicroElectroMechanical Systems Defense Advanced Research Projects Agency

BioMEMS Fabricated on Silicon Chip Interdigitated Array electrodes 100micrometers Fabricated on Silicon Chip Peristaltic Pumps Fluidics Layer Interdigitated Array electrodes Interdigitated Array electrodes- allow monitior cells metabolic activity by measuring the uptake of oxygen and glucose .25nL

Array Output of Intensity of Signal Project Design Light BioMEM Device, single cell w/dye Microscope DAQ Board Amplifier Labview Array Output of Intensity of Signal Voltage Photodiode Array

Project Overview Step 1: Design the hardware for a 256 channel data acquisition system to read out the Hamamatsu photodiode array Picture o f data acqusiiton board

Project Overview Step 2: Picture of LABVIEW Design Labview program to control the data acquisition system and provide control signals for the valves driving the bioMEMS device Picture of LABVIEW

Project Overview Step 3: Picture of LABVIEW Design Hardware to interface the photodiode array with an inverted microscope and the bioMEMS devices Picture of LABVIEW

Project Overview Step 4: Picture of LABVIEW Devise a calibration protocol for the photodiode array bandwidth of dye Picture of LABVIEW

Project Overview Step 5: Record transmembrane potential of single cardiac myocytes with flourescent probes and record from electrochemical sensors Flourescent probe= the measurement from the cell Electrochemical sensor= interdigitated array

Progress Research Darpa Proposal Journals, Internet etc Completed Steps 1, 2 Started Step 3: Modifications of Labview Program Collects in 2D array Need 3D Array (Data Organization) Testing to make sure that the gain is linear across all of the channels

3D Array

Future Plans Finish Step 3: Modify Labview program Organize data in 3D array Begin taking measurements