Presentation on Internship Program MD. MINHAZUR RAHMAN ID: 11336002

Acknowledgement Professor A.A.Z. Ahmad Chairperson, Department of Mathematics and natural Sciences, BRAC University Professor Naiyyum Choudhury Chairman, Bangladesh Atomic Energy Regulatory Authority Dr. Firdausi Qadri -Director of Centre for Vaccine Sciences, Senior Scientist & Head of Immunology Unit, icddr,b -Executive Director, ideSHi

Institutions Institute for Developing Science and Health Initiatives Immunology laboratory, The International Centre for Diarrheal Disease Research

Objectives Practical experience. Skill development. To be familiar with different environment. To work with the professional. .

Laboratory Learning

Icddr,b Identification of Vibrio cholerae and ETEC Identification and confirmation of V. cholerae by agglutination using Mab Dark field microscopy Dark field microscopy

Identification of Enterotoxigenic E. coli by PCR Template Preparation PCR Master Mix Gel electrophoresis to confirm the presence of toxin genes (HTS, HTP and LT) Gel electrophoresis

GM1 ELISA Detection of E. coli heat-stable (ST) and heat-labile toxins ELISA Test

LPS and CT ELISPOT Assay Detection of the frequency of immunoglobulin-secreting B cells. Quick Dot Blot Detection of Colonization factor (CF) by immuno dot blot assay Detection proteins on the bacterial cell wall. ELISPOT Assay

Flow Cytometry Detection of Human B and T cell by Flow Cytometry Cell counting, and protein engineering Fluorescent activated cell sorting machine

ideSHi TP test TP test using ELISA Isolation of peripheral blood mononuclear cells PBMCs Ficoll Gradient Centrifugation Method RBC Lysis Method

Genomic DNA extraction from human blood sample Blood collection Blood cell lysis Isolation of DNA PCR PCR master mix Thermal cycle Gel electrophoresis

Acute Respiratory and Enteric Infection diagnosis Isolation and identification of microorganisms Diagnosis protocols (pneumonia, diarrhea, typhoid) Gram staining and culture techniques. Uses of different media like blood agar media.

Learning … Using modern equipment for different diagnosis purpose How to work in a professional laboratory. How to set up a laboratory. How to work in groups and different people. how to maintain the rules regulation and the lab environment.

Thank you