1. Treatments that stimulate the E.coli. to take up foreign plasmids include: 1.CaCL2 treatment 2.Heat shock 3.Incubation with ECORI 4.1 and 2 5.2 and 3 6.All of the above

2. If transformation with the Pglo plasmid is successful, growth should occur on all plates except: 1.+DNA/lb/amp/ara 2.+DNA/lb/amp 3.-DNA/lb/amp 4.-DNA/lb

3. In the green fluorescent colonies, what is fluorescing? 1.The Pglo plasmid 2.The GFP 3.Arabinose 4.Uv light

4. What is the puropose of the arabinose in the Pglo transformation lab? 1.It make the E. coli. competent 2.It helpd the E.coli take up the plasmid 3.It turns on the GFP gene 4.It turns on the amp resistance gene 5.3 and 4

5. In what region of the lambda virus genome is gene H found? 1.Head proteins 2.Tail proteins 3.Attachment/integrat ion/excision 4.Control region 5.lysis

6. What is ECORI used for: 1.Making gels 2.Making E.coli competent 3.Digesting DNA 4.Turning on the H gene

7. A gene found on the PUC-18 plasmid not found on the Pglo plasmid: 1.Bla 2.Lac Z 3.ECORI 4.2 and 3

8. How many DNA fragments are produced why lambda DNA is digested with ECORI? 1.1 2.2 3.4 4.6 5.7

9. After colonies grow on the Xgal/amp agar plate, what do you do next with the colonies? 1.Transform them 2.Treat them with ECORI 3.Heat shock them 4.Treat them with CaCl2 5.Transfer them to a tube of broth

10. Lysozyme is used in: 1.Electrophoresis 2.Trasnformation 3.Plasmid isolation 4.Making recombinant PUC- 18 plasmids

11. What is agarose used for? 1. Making gels 2. Making E.coli competent 3. Digesting DNA 4.4. Turning on the H gene

12. What does electrophoresis sample buffer contain? 1.ECO RI 2.CaCL2 3.Tracking dye 4.Glycerol 5.2 and 3 6.3 and 4

13. The wells of the electrophoresis are placed nearest: 1.The negative terminal of the chamber 2.The positive terminal of the chamber

14. There are 4 DNA markers that we applied to our gel (784, 1120, 2010 and 3621). Which of these will migrate the furthest away from the wells during electrophoresis? 1.784 2.1120 3.2010 4.3621

15. In which ECORI fragment of lambda DNA do we expect to find the H gene? 1.3530 2.4878 3.5804 4.5643 5.7421 6.21226

16. Which colonies contain the plasmids that have a PUC- 18 plasmid with lambda DNA inserted into them ? 1.Blue colonies 2.White colonies 3.Green fluorescent colonies

17. In order to determine which lambda DNA fragment was inserted into cells from white colonies, which technique(s) did you do in lab? 1.Prepare recombinant PUC-18 /lambda DNA plasmids 2.Plasmid isolation 3.Electrophoresis 4.ECO RI digestion of recombinant PUC-18 /lambda DNA plasmids 5.3 and 4

18. Which of the following is the lambda genomic library? 1.The tube of recombinant PUC- 18/lambda DNA plasmids 2.The stained electrophoresis gel 3.The Xgal/amp agar plate with white colonies 4.The broth tube of a white colony from the X-gal/amp agar plate.