Date of download: 12/28/2017 Copyright © ASME. All rights reserved. From: Non-Hertzian Approach to Analyzing Mechanical Properties of Endothelial Cells Probed by Atomic Force Microscopy J Biomech Eng. 2005;128(2):176-184. doi:10.1115/1.2165690 Figure Legend: Contact mode AFM images (15×15μm) of untreated HAECs (A), (C), (D) and after 45min treatment with 4μM cytochalasin B (B). Superposed on each image is a gray scale map of the pointwise elastic modulus extracted at an indentation depth of 200nm obtained from an array of 64 force curves as described in Fig. with the region size and location drawn to scale. Histograms show the distribution of values from each corresponding modulus map, aligned with the scale bar for the modulus values (0–16kPa). Note alignment of stiff regions with fibrous structures in untreated cells, and a broad or bimodal distribution of modulus values compared to the cytochalasin-treated cell.