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Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Images of part of the erythrocytes under a differential interference contrast (DIC)

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Presentation on theme: "Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Images of part of the erythrocytes under a differential interference contrast (DIC)"— Presentation transcript:

1 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Images of part of the erythrocytes under a differential interference contrast (DIC) microscope cultured in (a) Li and (b) Pb ion solutions at 20, 40, 60, 80, 100, and 120 min, respectively. The black bar represents 20 μm. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

2 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. (a) DIC image of erythrocytes; (b) cell contours of erythrocytes extracted from (a) by automatic cell recognition; (c) mean cellular areas at different cultured time. At different times, 60 erythrocytes were used for statistical analysis. Error bars indicate standard deviation. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

3 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. (a) Scheme of the quantitative interferometric microscopy (QIM) system; (b) procedures of QIM including interferogram capturing, phase retrieval, cell recognition, and data statistical analysis; (c) phase steps; (d) AFM image of phase steps, where the white bar equals 10 μm; (e) retrieved height distribution from phase image. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

4 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. (a) Phase distribution of an erythrocyte (erythrocyte 1) in the 0.9% NaCl solution at beginning; (b) phase distribution of erythrocyte 1 captured after 120 min; (c) and (d) phase distribution of erythrocyte 2 captured at the beginning and after 120 min, respectively; (e) and (f) phase distribution of erythrocyte 3 at the beginning and after 120 min, respectively; (g) PA and PV comparisons of three erythrocytes; (h) and (i) DIC images at the beginning and after 120 min, respectively. (j) Cellular area comparisons between those at the beginning and after 120 min. Sixty (60) erythrocytes were used for each statistical analysis. Error bars in the figure indicate the standard deviation. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

5 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Quantitative phase distributions of an erythrocyte cultured in Li ion solution from 10 to 120 min with an interval of 10 min. The units of PA and PV are μm2 and rad μm2, respectively. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

6 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Quantitative phase distributions of erythrocytes cultured in Li ion solution at 10, 50, and 100 min, respectively. The units of PA and PV are μm2 and rad μm2, respectively. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

7 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Quantitative phase distributions of an erythrocyte cultured in Pb ion solution from 10 to 120 min with an interval of 10 min. The units of PA and PV are μm2 and rad μm2, respectively. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

8 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Quantitative phase distributions of erythrocytes cultured in Pb ion solution at 10, 50, and 100 min, respectively. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105

9 Date of download: 5/28/2016 Copyright © 2016 SPIE. All rights reserved. Quantitative PA and PV comparisons of erythrocytes cultured in Li and Pb ion solutions. In both Li and Pb cases, 40 erythrocytes were used for statistical analysis. Error bars indicate standard deviation. Figure Legend: From: Phase measurements of erythrocytes affected by metal ions with quantitative interferometric microscopy Opt. Eng. 2015;54(12):124105. doi:10.1117/1.OE.54.12.124105


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