Francisella tularensis V. Mosley F2013 Modified by DYH Gram negative Small, pleomorphic coccobacillus Encapsulated non-motile causes tularemia also called glandular fever, rabbit fever, tick fever, and deer fly fever Infects through the bite of infected insects (ex. ticks and deerflies) by handling infected sick or dead animals (ex. rabbits, rodents, and hares) by eating or drinking contaminated food or water by inhaling airborne bacteria Table 1 Overall features of the genome of F. tularensis strain SCHU S4 Size 1,892,819 bp G+C content (%) 32.9 CDS 1,804 Coding percentage 79.4 Unique genes 302 Pseudogenes or gene fragments 201 IS elements 74   ISFtu1 (IS630 family) 50   ISFtu2 (IS5 family) 16   ISFtu3 (ISHpaI-IS1016 family) 3   ISFtu4 (IS982 family) 1   ISFtu5 (IS4 family) rRNAs 3 operons tRNAs 38 Other stable RNAs 7 Subspecies (Human isolate) http://www.nature.com/ng/journal/v37/n2/full/ng1499.html buffered charcoal yeast extract; (B) chocolate agar medium; (C) sheep’s blood agar; (D) cysteine heart agar. http://www.ppdictionary.com/bacteria/gnbac/tularensis.htm

Francisella tularensis V. Mosley F2013 Modified by DYH Virulence factors not well studied hemolytic agents may facilitate degradation of the phagosome NlyA (hemolysin activity ) ATP binding cassette (ABC) proteins may be linked to the secretion of virulence factors type IV pili bind to the exterior of a host cell thus become phagocytosed IglC (23-kD protein ) phagosomal breakout and intracellular replication Down regulates immune response to increase growth time AcpA inhibts the respiratory burst No natural resistance to antibiotics used to treat the diesease Tularemia. Number of reported cases - United States and U.S. territories, 2007 http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5653a1.htm