Diagnosis of autoimmune pancreatitis by EUS-guided FNA using a 22-gauge needle: a prospective multicenter study  Atsushi Kanno, MD, PhD, Atsushi Masamune,

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Diagnosis of autoimmune pancreatitis by EUS-guided FNA using a 22-gauge needle: a prospective multicenter study  Atsushi Kanno, MD, PhD, Atsushi Masamune, MD, PhD, Fumiyoshi Fujishima, MD, PhD, Takuji Iwashita, MD, PhD, Yuzo Kodama, MD, PhD, Akio Katanuma, MD, Hirotaka Ohara, MD, PhD, Masayuki Kitano, MD, PhD, Hiroyuki Inoue, MD, PhD, Takao Itoi, MD, PhD, Nobumasa Mizuno, MD, PhD, Hiroyuki Miyakawa, MD, PhD, Rintaro Mikata, MD, PhD, Atsushi Irisawa, MD, PhD, Satoko Sato, MD, PhD, Kenji Notohara, MD, PhD, Tooru Shimosegawa, MD, PhD  Gastrointestinal Endoscopy  Volume 84, Issue 5, Pages 797-804.e1 (November 2016) DOI: 10.1016/j.gie.2016.03.1511 Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 1 Macroscopic findings of the specimens obtained by EUS-FNA with a 22G needle demonstrated adequate specimens for histopathologic diagnosis (H&E, orig. mag. ×10). Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 2 The findings in high-power fields (H&E, orig. mag. ×400) reveal abundant lymphoplasmacytic infiltration. Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 3 IgG4 immunostaining in the specimens obtained by EUS-FNA shows markedly increased numbers of IgG4-positive plasma cells (orig. mag. ×400). Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 4 Immunohistochemical staining of CD38. Abundant CD38-positive plasma cells were found in the high-power field (orig. mag. ×400). Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 5 A specimen showing storiform fibrosis in high-power fields (H&E, orig. mag. ×400). Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 6 Obstructive phlebitis revealed by (A) H&E and (B) Elastica-Masson staining (orig. mag. ×200) shows that the infiltration of inflammatory cells obstructed the vein. Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions

Figure 7 Flowchart illustrating the summary of this study focusing on the diagnosis according to the ICDC. AIP, autoimmune pancreatitis; OOI, other organ involvement; ICDC, International consensus diagnostic criteria. Gastrointestinal Endoscopy 2016 84, 797-804.e1DOI: (10.1016/j.gie.2016.03.1511) Copyright © 2016 American Society for Gastrointestinal Endoscopy Terms and Conditions