Cell Counts by Hemocytometer

9 large squares – 1mm x 1mm Center square divided into 25 smaller squares - 0.2mm x 0.2mm = 0.04 mm2 Depth of the counting chamber – 0.1 mm Volume of a small square – 0.04 mm2 x 0.1 mm = 0.004 mm3 1 ml = 1 cm3 = 1000 mm3 (10 mm = 1 cm)

40x magnification 1 mm

100x magnification 0.2 mm

400x magnification

Zoox Density Calculations Average of 10 counts of 0.004 mm3 = 6 zoox 0.004 mm3 x 1 ml/1000mm3 = 4 x 10-6 ml 6 zoox/4 x 10-6 ml = 1.5 x 106 zoox/ml Total homogenate volume = 5 ml Total zoox = 5ml x 1.5 x 106 zoox/ml = 7.5 x 106 zoox Wet weight of polyps = 0.25 g Zoox density: 7.5 x 106 zoox/0.25 g wet wt. = 3.0 x 107 zoox/g wet wt.