Creative Filtration and Chromatography Products Luknova, Inc. 20 Cabot Blvd., Suite 300, Mansfield, MA 02048, USA Confidential
About Luknova Incorporated in Princeton, New Jersey (Feb. 2004) R&D started from the Advanced Technology & Manufacturing Center (ATMC), University of Massachusetts, Dartmouth Relocated to Boston (2009) Designed in Massachusetts by Luknova Manufactured in both U.S. and overseas. Confidential
Products - EasyFil™ filters Absolutely leak free design with ground joint – built-in peace of mind in filtration Confidential
Products - EasyFil™ filters Three welded-seals and one bump seal - absolutely leak-free Confidential
Products - EasyFil™ filters Proprietary products (patents spending) All made with polymer Safe, not fragile Leak-free Faster filtration High yield Disposable Reusable Adjustable long stem - No splashing and No molecular damage Confidential
Products - EasyFil™ filters Application advantages Speed, faster separation Save time, save solvent No contamination, disposable No bypass leakage No sample-loss to strong vacuum No splashing No molecular damage Adjustable long- and short-stem Reusable (>20 times) Construction Characs Polypropylene (PP) housing Polyethylene (PE) frit (pore size 10µm) Multiple welded-seals to prevent leakage All parts are welded together as one piece Recommended max temperature 110oC 14/20, 14/35, 24/25, and 24/40 standard joints Confidential
Products - EasyFil™ filters Solvent test We tested a variety of common solvents and found there are no apparent changes at following conditions: at the boiling points of Ethyl acetate , DMF, DMA, diethyl ether, dichloromethane, THF, hexane, and water; at concentrated H2SO4, HCl, HBr, HI, acetic acid, and TFA; at saturated NaOH and KOH solution; DMSO, at temperature of 120oC (not tested above 120oC) Confidential
Flash columns Confidential
Flash Columns – Design Five piece composition a perforated cap a sealing insert an inlet filter an outlet filter a column body Features Easy assembly and disassembly Easy sample recovery and load Easy sorbent packing/repacking Disposable and reusable Confidential
Flash Columns – Performance Date: March 6, 2009 Column: Luknova 40g silica Gradient: 0 to 50% EA/Hexane Run length: 15 min Detection wavelength: 254 nm Monitor wavelength: 280 nm Flow rate: 40 mL/min Equilibration volume: 235 mL Initial waste: 0 mL Column: Luknova 40g prepacked standard flash column. Sample: A total volume 390 µL of 1:1:1 ratio of toluene, phthalic acid dimethyl ester, and phthalic acid diethyl ester mixed with 500µL methylene chloride. Solvent: Hexane (A) and Ethyl Acetate (B). Instrument: CombiFlash system. Confidential
Performance – Luknova vs. ISCO Luknova column did reach baseline separation Isco RF column ISCO column did not reach baseline separation Confidential
Products – QC Control Luknova’s 5-Component QC Standard Accurate Results Quicker More efficient Symmetric peaks Sharper peaks No fronting No doubling No overlapping High yield No compound decomposition Reliable purification Consistent performance Confidential
A variety of flash columns Silica columns 4g-1500g 50 and 25µm particles Specialty columns Amine, Cyano, Diol, Florisil, Alumina (basic, neutral, acid) C4, C8, C18, C30, Cyclohexyl, Phenyl WCX, SCX, SCX-2, SAX, and WAX Mixed phases Confidential