1. Chapter 9 Column Chromatography

2. Column Chromatography (CC)
Column chromatography is a useful method for separation and purification of solids and liquid samples under 10 grams.
CC works on the same principle of TLC except that the sample solution flows down a column of silica gel or alumina filled with solvent.
A partition coefficient represents the efficiency of the column and is determined by the solubility of the solute in the two phases.

3. Column Chromatography (CC)
Only complete Part 1 (microscale) pp , Ferrocene and Acetylferrocene.
Packing the Column
Make sure your column has a polyethylene frit.
Pour the silica gel (or alumina) into the column so it is 1/2 to 2/3 full.
Pour this silica gel into a 50 mL beaker and add 8 mL of hexane.
Clamp the column in a vertical position.
Add 2 mL of hexane to the column.
Stir the hexane silica gel mixture while pouring into the column.
Open the valve and drain the solvent. Do not let the solvent level get below the silica gel.
Repeat the last three steps until all of the silica gel is in the column.
Close the valve with some solvent remaining at the top of the column.

4. Column Chromatography (CC)
Follow the handout for further experimental steps and obtain the following:
Weight of crude ferrocene and of crude acetylferrocene.
Melting points of ferrocene and of acetylferrocene.
Percent Recovery

5. Comments Acetylferrocene is toxic. Handle with care.
Hexane is flammable. Be aware of your surroundings.
It should be easy to follow this separation process as both compounds are colored.