Electron Microscope Dr. Laxmi Kant Pandey.

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Presentation transcript:

Electron Microscope Dr. Laxmi Kant Pandey

Introduction An electron microscope is a microscope that uses a beam of accelerated electrons (From tungsten filament) as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.

electron microscopes use electromagnetic lenses to control the electron beam and focus it to form an image. Electron microscopes are used to investigate the ultrastructure of a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, metals, and crystals. Modern electron microscopes produce electron micrographs using specialized digital cameras.

Types TEM (Transmission Electron Microscope) SEM (Scanning electron Microscope)

High voltage Phosphor Zinc Sulphide

Application: Topography Particle analysis Particle detection Protein localization Structural biology Virology (e.g. viral load monitoring

high-resolution transmission electron microscopy (HRTEM) has allowed the production of images with resolution below 0.5 angstrom (50 picometres)[1] and magnifications above 50 million times.[10] The ability to determine the positions of atoms within materials has made the HRTEM an important tool for nano-technologies research and development