Cardiac Morphology, Gene Expression and Histology (Core D)

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Cardiac Morphology, Gene Expression and Histology (Core D)

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Cardiac Morphology, Gene Expression and Histology (Core D) Description of Core Unit: Generate staged mouse and Xenopus embryo RNA samples. Generate paraffin and OCT embedded tissues and embryos. Share expertise in and perform some whole mount and section in situ hybridizations. Perform IHC and histology on tissue and embryo sections.

Core D Progress Report I Successful use of RT-PCR to verify embryonic expression of Xenopus Mo2L mRNA (Tiziana De Angelis). Next: in situ hybridizations (ISH; mouse and Xenopus; Projects 4 & 5, Ning Dai and Tiziana De Angelis) including Mo2L and mouse Ehmt1 (Project 4). Control probes for mouse in situs obtained from Tom Lufkin. Successful sub-cloning of Xenopus LKB1/XEEK1 in KmRN3P.

Core D Progress Report II Additional histological materials generated to date (including NIH Progress Report): 1518 slides stained. 1947 slides unprocessed. Total no. of slides: 3465. 245 embedded tissue blocks. 239 processed blocks. Total no. of blocks: 484. Ning Dai

Interactions with other projects are welcome.