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Supplementary figures Supplementary Fig. 1. Inhibition of PKA by the dominant negative PKA (dnPKA) did not block the isoproterenol-induced decrease of p300. H1299 cells were transfected with dnPKA and incubated for 24 h before treatment with 1 mM isoproterenol (IPO) for 30 min. Asterisks (*) indicate a statistically significant difference from the vector-transfected control; the double asterisks (**) represent a statistically significant difference from the isoproterenol-treated control. Supplementary Fig. 2. Inhibition of Epac by brefelden A (BFA) blocked the isoproterenol-induced decrease of p300. H1299 cells were treated with 1 mM isoproterenol (IPO) and 10 mg/ml of BFA for 30 min. Asterisks (*) indicate a statistically significant difference from the vehicle-treated control; the double asterisks (**) represent a statistically significant difference from the isoproterenol-treated control. Supplementary Fig. 3. (A) Effect of isoproterenol and 8-pCPT-cAMP on MAPKs. H1299 cells were treated with 1 mM isoproterenol (IPO) or 20 mM 8-pCPT-cAMP for 30 min before being analysed by western blotting.

Supplementary Fig 1 * * ** p300 p-CREB b-Actin IPO - + dnPKA V

Supplementary Fig 2 ** * p300 p-CREB b-Actin IPO - + BFA

Supplementary Fig 3 p300 p-ERK ERK p-JNK JNK p-p38 p38 p-AKT AKT b-Actin IPO - + 8-pCPT