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Figure 1. Summary of PTH secretion from human parathyroid cells after caffeine treatment at concentrations of 0, 1, 10, and 50 μm at extracellular calcium.

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Presentation on theme: "Figure 1. Summary of PTH secretion from human parathyroid cells after caffeine treatment at concentrations of 0, 1, 10, and 50 μm at extracellular calcium."— Presentation transcript:

1 Figure 1. Summary of PTH secretion from human parathyroid cells after caffeine treatment at concentrations of 0, 1, 10, and 50 μm at extracellular calcium ([Ca<sup>2+</sup>]<sub>e</sub>) of 1.25 mm. The PTH level without caffeine was set at 1.0 for each experiment; 0.5 and 1.8 mm Ca<sup>2+</sup> were applied as controls for cell responsiveness. n, number of experiments. Box-plots show median levels together with 10th, 25th, 75th, and 90th percentiles, outline indicated as open circle. *, P < .05 as compared to control cells. From: Inhibition of Parathyroid Hormone Secretion by Caffeine in Human Parathyroid Cells J Clin Endocrinol Metab. 2013;98(8):E1345-E1351. doi: /jc J Clin Endocrinol Metab | Copyright © 2013 by The Endocrine Society

2 Figure 2. Measurement of [Ca<sup>2+</sup>]<sub>i</sub> using Fura-2 after application of 50, 200, or 500 μm caffeine (A) and 10 μm and 5 mm caffeine (B). From: Inhibition of Parathyroid Hormone Secretion by Caffeine in Human Parathyroid Cells J Clin Endocrinol Metab. 2013;98(8):E1345-E1351. doi: /jc J Clin Endocrinol Metab | Copyright © 2013 by The Endocrine Society

3 Figure 3. Protein expression of ADORA<sub>1</sub> and ADORA<sub>2A</sub> in parathyroid tissues. A, Autoradiograms of Western blot analyses for protein expression of ADORA<sub>1</sub> and ADORA<sub>2A</sub> in 7 human parathyroid adenomas, normal parathyroid tissue, and human glioblastoma U251 cells used as positive control. Subsequent incubation with anti-GAPDH was used as protein-loading control. B, Photomicrographs showing ADORA<sub>1</sub> expression in parathyroid adenoma and in the adjacent rim of normal parathyroid (magnification, ×36). From: Inhibition of Parathyroid Hormone Secretion by Caffeine in Human Parathyroid Cells J Clin Endocrinol Metab. 2013;98(8):E1345-E1351. doi: /jc J Clin Endocrinol Metab | Copyright © 2013 by The Endocrine Society

4 Figure 4. Relative mRNA expression levels of PTH, ADORA1, and ADORA2A determined by qRT-PCR after incubation with 0, 1, 10, or 50 μm caffeine. The expression level in the noncaffeine control was set at the arbitrary value of 1.0 for each experiment. Box-plots show median, 10th, 25th, 75th, and 90th percentiles. *, P < .05 as compared to control cells. From: Inhibition of Parathyroid Hormone Secretion by Caffeine in Human Parathyroid Cells J Clin Endocrinol Metab. 2013;98(8):E1345-E1351. doi: /jc J Clin Endocrinol Metab | Copyright © 2013 by The Endocrine Society

5 Figure 5. Results from measurements of intracellular cAMP and PKA in human parathyroid adenoma cells exposed to caffeine for 30 minutes. A, Intracellular cAMP measured after administration of caffeine at concentrations of 1, 10, 50 μm and 5 mm, and for control cells without caffeine treatment. B, PKA activities after incubation with 0, 1, 10, or 50 μm caffeine. Intracellular cAMP and PKA activity of the noncaffeine control was set at 1.0 for each experiment. Box-plots show median levels together with 10th, 25th, 75th, and 90th percentiles, outline indicated as open circle. *, P < .05 as compared to control cells. From: Inhibition of Parathyroid Hormone Secretion by Caffeine in Human Parathyroid Cells J Clin Endocrinol Metab. 2013;98(8):E1345-E1351. doi: /jc J Clin Endocrinol Metab | Copyright © 2013 by The Endocrine Society

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