The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: A potential mechanism for senescence in.

Slides:

Advertisements

Similar presentations

Timing of carotid endarterectomy after acute stroke Joseph J. Plotrowski, MD, Victor M. Bernhard, MD, Jeffrey R. Rubin, MD, Kenneth E. McIntyre, MD, James.

Advertisements

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

Professionalism and the work-life balance Thomas S. Huber, MD, PhD Journal of Vascular Surgery Volume 60, Issue 4, Pages (October 2014) DOI:

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

Clinical evaluation of recombinant human platelet – derived growth factor for the treatment of lower extremity diabetic ulcers David L. Steed, MD, the.

Aortic injury occurring after minor trauma in ankylosing spondylitis

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix Sung Shin, MD, Yong-Pil.

Impact of a critical pathway on postoperative length of stay and outcomes after infrainguinal bypass Andrew C. Stanley, MD, Maryann Barry, RN, Thayer.

Presidential address: Energy, poise, and resilience—Daniel Bernoulli, Thomas Young, J. L. M. Poiseuille, and F. A. Simeone Robert W. Hopkins, MD Journal.

Scott D. Gitlin, MD, Michael Deeb, MD, Christopher Yann, BS, Alvin H

Mycotic axillary artery aneurysm

A new valvulotome for in situ bypass grafts

Aortic injury occurring after minor trauma in ankylosing spondylitis

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD

Altered proliferative responses of dermal fibroblasts to TGF-β1 may contribute to chronic venous stasis ulcer1 Brajesh K Lal, MD, Satoshi Saito, MD,

The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: A potential mechanism for senescence in.

Ambulatory venous pressure revisited

Malcolm O. Perry, MD, Richard Kempczinski, MD

Recurrent thoracic outlet syndrome

Protecting the brain and spinal cord

Healing rates and cost efficacy of outpatient compression treatment for leg ulcers associated with venous insufficiency William A. Marston, MD, Robert.

A prospective, randomized trial of Unna's boot versus Duoderm CGF hydroactive dressing plus compression in the management of venous leg ulcers Paul R.

Limb salvage and wound coverage in patients with large ischemic ulcers: A multidisciplinary approach with revascularization and free tissue transfer

Carotid artery replacement in conjunction with resection of squamous cell carcinoma of the neck: Preliminary results M.Kathleen Reilly, PhD, MD, Malcolm.

Venous photoplethysmography: Relationship between transducer position and regional distribution of venous insufficiency Stefan Rosfors, MD Journal of.

Skin perfusion pressure in the prediction of healing in diabetic patients with ulcers or gangrene of the foot I. Faris, M.D., F.R.A.C.S., H. Duncan,

Giancarlo Piano, MD, Bruce L. Gewertz, MD Journal of Vascular Surgery

Reduction of hospital resources utilization in vascular surgery: A four-year experience Sean P. Roddy, MD, Thomas F. O'Donnell, MD, Mark D. Iafrati, MD,

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

Objective tinnitus resulting from internal carotid artery stenosis

Transforming growth factor-β1 increases lysyl oxidase enzyme activity and mRNA in rat aortic smooth muscle cells Charles J. Shanley, MD, Mehrnaz Gharaee-Kermani,

Primary leiomyosarcoma of the abdominal aorta

The diameter of the common femoral artery in healthy human: Influence of sex, age, and body size Thomas Sandgren, MD, Björn Sonesson, MD, PhD, Åsa Rydén.

The effect of carbon coating and porosity on early patency of expanded polytetrafluoroethylene grafts: An experimental study Donald L. Akers, MD, Yong.

Treatment of venous disease – The innovators

A. N. Nicolaides, MS, FRCS, M. K. Hussein, MD, FRCS, G. Szendro, MD, D

Functional analysis of cryopreserved veins

Lower extremity ischemia in adults younger than forty years of age: A community-wide survey of premature atherosclerotic arterial disease Pavel J. Levy,

Calvin B. Ernst, MD Journal of Vascular Surgery

Ruptured abdominal aortic aneurysm: A population-based study

Mesenteric venous thrombosis

Keratinocytes Inhibit Expression of Connective Tissue Growth Factor in Fibroblasts In Vitro by an Interleukin-1α-Dependent Mechanism Daniel Nowinski,

Cardiovascular surgery—The rocket and its stars: Presidential address

Composite sequential bypasses to the ankle and beyond for limb salvage

Ambulatory venous pressure revisited

Research and research funding in vascular surgery

Regarding “Venous ulcers and the superficial venous system”

J.Dennis Baker, MD Journal of Vascular Surgery

Regional nerve block for femoropopliteal and tibial arterial reconstructions Joseph M. Giordano, M.D., George A. Morales, M.D., Hugh H. Trout, M.D., Ralph.

Septic embolism complicating infective endocarditis

William H. Pearce, MD, Iliana Sweis, MD, James S. T. Yao, MD, Walter J

Seizures following subclavian-carotid bypass

Jay B. Fisher, MD, Marc A. Granson, MD Journal of Vascular Surgery

Hemodynamic basis of stasis ulceration—A hypothesis

Biochemical analysis of a human humoral fibroblast inhibitory factor associated with impaired vascular prosthetic graft incorporation Jacob Schneiderman,

Evaluation of a proposed standard reporting system for preoperative angiograms in infrainguinal bypass procedures: Angiographic correlates of measured.

Exposure of femoral vessels by a suprainguinal incision

Monitoring vascular surgical performance

Aortoesophageal fistula: Case report and literature review

D. Emerick Szilagyi, MD—An appreciation

Presidential address: The second-generation vascular surgeon

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Fibroblasts cultured from venous ulcers display cellular characteristics of senescence Manuel V. Mendez, MD, Andrew Stanley, MD, Hee-Young Park, PhD,

Jerry Goldstone, MD Journal of Vascular Surgery

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease Joseph P. Archie, PhD, MD Journal of Vascular.

Popliteal entrapment syndrome: A report of tibial nerve entrapment

Presentation transcript:

The proliferative capacity of neonatal skin fibroblasts is reduced after exposure to venous ulcer wound fluid: A potential mechanism for senescence in venous ulcers Manuel V. Mendez, MD, Joseph D. Raffetto, MD, Tania Phillips, MD, James O. Menzoian, MD, Hee-Young Park, PhD Journal of Vascular Surgery Volume 30, Issue 4, Pages 734-743 (October 1999) DOI: 10.1016/S0741-5214(99)70113-8 Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Representative sample of growth curves of neonatal foreskin fibroblasts cultured in the presence of complete media (squares ) and in the presence of venous ulcer wound fluid (triangles ). The best-fit line for each curve was calculated by regression analysis and is shown for each curve. The slope of this line (dotted line) was then calculated and used as an estimate of the growth rate. Journal of Vascular Surgery 1999 30, 734-743DOI: (10.1016/S0741-5214(99)70113-8) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Neonatal foreskin fibroblast growth rates in the presence or absence of venous ulcer wound fluid (VUWF). The VUWF bar represents the mean ± SEM of four different samples tested. CM, Complete media; asterisk, P = .006 Journal of Vascular Surgery 1999 30, 734-743DOI: (10.1016/S0741-5214(99)70113-8) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Neonatal foreskin fibroblast growth rates (± SEM) when culture in the presence of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, and interleukin (IL)-1β. Dose-response curves were performed for each cytokine. Each data point represents the growth rate derived by calculating the slope of a 3-point growth curve. Data points in each growth curve derive from duplicate samples. Journal of Vascular Surgery 1999 30, 734-743DOI: (10.1016/S0741-5214(99)70113-8) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Neonatal foreskin fibroblast senescence associated β-galactosidase activity (SA-β-Gal) activity after 12 days in culture in the presence of venous ulcer wound fluid (VUWF) and pro-inflamatory cytokines. The VUWF bar represents the mean ± SEM of four different VUWF samples tested. The tumor necrosis factor (TNF)-α bar represents the mean ± SEM of four independent observations. CM, Complete media; TGF-β, tranforming growth factor-β; IL-1β, interleukin-1β; asterisk, P < .03 when compared to control (CM). Journal of Vascular Surgery 1999 30, 734-743DOI: (10.1016/S0741-5214(99)70113-8) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Representative photomicrographs of neonatal foreskin fibroblasts (NFFs) under light microscopy at ×10 (1,2,3) and ×25 (4) magnification. All photomicrographs were obtained from tissue culture plates subjected to the same culture conditions and on day 7 after initial seeding (500 cells/plate). 1, NFFs cultured in the presence of complete media (CM) alone. 2, NFFs in the presence of CM and venous ulcer wound fluid (VUWF). 3 and 4, NFFs in the presence of TNF-α. The arrows mark fibroblasts with the typical morphologic features of senescence. Journal of Vascular Surgery 1999 30, 734-743DOI: (10.1016/S0741-5214(99)70113-8) Copyright © 1999 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions