1. Biochemical analysis of a human humoral fibroblast inhibitory factor associated with impaired vascular prosthetic graft incorporation 
Jacob Schneiderman, MD, Sarah Knoller, PhD, Raphael Adar, MD, Naphtali Savion, PhD 
Journal of Vascular Surgery 
Volume 14, Issue 1, Pages (July 1991)
DOI: / (91)90161-M
Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2. Fig. 1
Morphology of HSF and capillary endothelial cells exposed to the inhibitory and normal serum. HSF cells (A, B) and bovine brain capillary endothelial cells (C, D) were grown in the presence of 50% and 25% serum, respectively. A and C were exposed to normal human serum; B and D were exposed to the inhibitory serum. Cultures were photographed on day 6 (Original magnification × 100).
Journal of Vascular Surgery  , DOI: ( / (91)90161-M)
Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3. Fig. 2
Purification of the inhibitory activity by sepharose 6B column. Inhibitory and NHS were precipitated with ammonium sulfate (25%), dissolved, dialyzed and applied to a sepharose 6B column. Fractions were collected, and absorbance at 280 nm was determined (solid line). The fractions were pooled, concentrated, and their effect on the proliferation of HSF cells at a final concentration of 25% was studied (bars). The pool of tubes was concentrated and reapplied to the column. Fractions at 5% final concentration were assessed for inhibitory activity (insert). The column was calibrated by use of molecular mass standards as indicated on the upper part of the figure.
Journal of Vascular Surgery  , DOI: ( / (91)90161-M)
Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4. Fig. 3
Gel electrophoresis of the inhibitory fraction. Lanes 1-4 are samples derived from NHS and lanes 5-8 are samples derived from the inhibitory serum. Lanes 1 and 5, fractions 34-35; Lanes 2 and 6, fractions 36-37; Lanes 3 and 7, fractions 38-39; Lanes 4 and 8, fractions Molecular mass standards are marked on the left side of the gel.
Journal of Vascular Surgery  , DOI: ( / (91)90161-M)
Copyright © 1991 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions