Adsorption and function of recombinant Factor VIII at the air–water interface in the presence of Tween 80 Omkar Joshi, Liping Chu, Joseph McGuire, D.Q.

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Presentation transcript:

Adsorption and function of recombinant Factor VIII at the air–water interface in the presence of Tween 80 Omkar Joshi, Liping Chu, Joseph McGuire, D.Q. Wang Journal of Pharmaceutical Sciences Volume 98, Issue 9, Pages 3099-3107 (September 2009) DOI: 10.1002/jps.21569 Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 1 Surface tension kinetics exhibited by rFVIII–Tween mixtures as a function of Tween concentration. rFVIII concentration is 24.5 µg/mL in each case. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 2 Steady state values of surface tension recorded for rFVIII–Tween mixtures plotted as a function of Tween concentration. The regions I–V are indicated based on trends empirically observed for simple protein–surfactant mixtures. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 3 Surface tension kinetics exhibited by Tween as a function of concentration. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 4 Steady state values of surface tension recorded for rFVIII–Tween mixtures as compared with protein-free solutions containing Tween as the only surface active species. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 5 Turbidity (A350 nm) data recorded for agitated rFVIII samples. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 6 Wavelength at maximum fluorescence emission recorded for rFVIII–Tween samples (a) exposed to the air–water interface through agitation, and (b) stored at room temperature without agitation. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 7 Normalized fluorescence intensity at 334 nm for (a) agitated, and (b) unagitated rFVIII–Tween samples. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions

Figure 8 Biological activity of Tween-containing rFVIII samples upon exposure to the air–water interface through agitation, and stored at room temperature without agitation. Journal of Pharmaceutical Sciences 2009 98, 3099-3107DOI: (10.1002/jps.21569) Copyright © 2010 Wiley-Liss, Inc. Terms and Conditions