Biotech Lab Safety Specifics Abbrevieations: PPE – Personal protective equipment Should be chosen based on specific risks Glasses with side protection if low splash risk Chemical goggles if large splash risk UV glasses with full face shield if short wave UV is used to view DNA banding Other example: aprons, lab coats, gloves, face masks and shoe covers

MSDS – Material Safety Data Sheet Lists potential hazards associated with the chemicals used in a laboratory

Biological Safety Level BSE 1 – Not a specific threat to handling individual All HS labs and most college labs BSE 2 - Moderate threat to individuals and the environment Some advanced college labs BSE 3 & 4 - pose a threat too great to for use an educational environment The amount of government regulation goes up with each level

General Laboratory Hazard Signs Harmful Explosive Corrosive Dangerous for the environment Flammable Toxic Oxidizing Radioactive Biohazard Poison General danger Electrical hazard

Disposal of Biohazardous Material Autoclave Uses pressure to increase the boiling point of water to 121oC 10% Bleach for countertops Must sit for 10min to be effective Recommended after each biological lab

Volumetric Equipment Most Common Graduated Flask Graduated Cylinder Erlenmeyer Flask Reagent Bottle

Pipette Types Volumetric … has graduations Serologic … has calibrated tip Pasteur (transfer) … made of glass & does not have graduations Used to transfer liquid from one container to another Disposable transfer pipette Standard thin stemmed plastic pipette