Fig. 4. Migration assays in neonatal normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs). NHKs (A) and HDFs (B) were cultured in either.

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Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **

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Fig. 4. Migration assays in neonatal normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs). NHKs (A) and HDFs (B) were cultured in either Wnt5a-conditioned or control media for 3 d, following which the cells (A: 1×106, B: 5×104) were incubated on transwell membranes (8-µm pore size) for 24 h. Migrated cells were stained with hematoxylin and eosin, and then enumerated under a microscope (×100). Fig. 4. Migration assays in neonatal normal human keratinocytes (NHKs) and human dermal fibroblasts (HDFs). NHKs (A) and HDFs (B) were cultured in either Wnt5a-conditioned or control media for 3 d, following which the cells (A: 1×106, B: 5×104) were incubated on transwell membranes (8-µm pore size) for 24 h. Migrated cells were stained with hematoxylin and eosin, and then. . . Ann Dermatol. 2016 Feb;28(1):45-54. http://dx.doi.org/10.5021/ad.2016.28.1.45