GUSTOR BP70

Butyric acid

Butyric acid ..short chain fatty acid resulting from microbial fermentation … • naturally present in the intestine of animals • provides antimicrobial activity • enhances villi and crypt development - high in caloric content - readily metabolized by intestinal epithelium - preferentially oxidized by colonocytes • anti-inflammatory properties Ebullition point for butyric? Butyric acid is produced within the animal from CHO (starch) escaping from digestion, so it is present in the intestine of fish. Butyric acid is well known, as other organic acids, due to its antimicorbial activity, however, it is also know by its important function in intestinal growth, regeneration and nutrient absorption. So it is essential for the development of epithelial cells due to some characteristics as being high in caloric content, beingreadily metabolized by the intestinal epithelium and being preferiantially oxidized by the colonocytes. On the top of that, , butyric acid has antinflammatory properties. However, butyric acid is volatile and corrosive, so when thinking in a practical way of feeding…. Comments: It is used as energy source for enterocytes (favourite energy source for enterocytes), increasing the development of villi. It has been associated with the protection against colon cancer and colon ulcers. Preferiantially oxidized when compared with common fuels (butyrate more than acetoacetate more than glutamine more than glucose) SFCA may normally supply 5-10% of daily energy requirement. it is known for short-chain fatty acids to be present in the fermentation in the intestine of herbivorous fish, but also in the intestines of carnivorous fish like "red sea bream" (Chrysophrys major). In salmon (Salmo salar) it has been established that such bacterial fermentation is much lower and that there is little of short-chain fatty acids in the intestine • volatile and corrosive (in feed)  Use of salts

Sodium butyrate as a source of energy for the intestinal mucosa

Sodium butyrate stimulates the gastrointestinal cell growth, acting as the main nutrient of enterocytes. Enterocytes: The better size, length and health, the greater the absorption of the nutrients. Glucose absorption Villus height (mm) One of the unique properties of sodium butyrate when compared to classic acidifiers is that it is a preferred source of energy for the enterocytes = the cells that are on the surface of the villi When villi are longer it increases the surface for absorption of nutrients Pluske et al., 1996

Effects of sodium butyrate on micro flora of small intestine of piglets after weaning at 6.7 kg live weight (duration of the trial 30 days) - Lu et al., 2008 Same observations as before, Bifidobacteria nd Lactobacilli develop more in presence of sodium butyrate while pathogens are reduced Please note that we have similar effect for 500 g and 1 kg per MT

GUSTOR BP70: 40% free Na But + 30% protected Na-But Vegetable Fat protection allows Butyrate to reach the distal parts of the GIT Free Butyrate acts in 1st half GIT Protected Butyrate acts in 2nd half GIT

Effect of Sodium Butyrate in layers van Eys, 2008 Better production due to less damage of intestinal wall Na-But ppm Laying % Egg Wt g Egg M g/h/d ADFI FCR 84.3 66.4 56.0 119.1 2.13 50 84.7 66.5 56.3 119.0 2.12 100 85.2 66.2 56.4 118.9 2.11 250 86.7 57.7 119.6 2.08 500 88.0 65.7 57.8 120.2

Effect of Sodium Butyrate in layers from 54 to 58 weeks of age van Eys, 2008

Effect of Sodium Butyrate on egg quality Sanchez et al., 2009

Effect of Sodium Butyrate on villi length (mm) in layers + 15% + 6% Sanchez et al., 2009

Effect of Butyrate in layers (Bovans, 63 weeks of age) Sanchez et al., 2009

Gustor in layers & breeders Effect on egg quality

Effect of Sodium Butyrate on egg quality Sanchez et al., 2009

Effect of Sodium Butyrate (250 g/Ton) in ISA breeders 28 weeks old AVPA, 2006

Gustor in layers & breeders Effect on mortality & hatchability

Effect of Sodium Butyrate (250 g/Ton) in ISA breeders 28 weeks old AVPA, 2006 Weeks 28 31 35 42

Effect of Butyrate/Lactate in breeders Bovans 75 weeks old Sengor et al., 2007