Table A. Sequences used in making CsKASII RNAi constructs

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Table A. Sequences used in making CsKASII RNAi constructs S1 File Table A. Sequences used in making CsKASII RNAi constructs 169 bp 5’ UTR sequence for RNAi-1 TTTTACGCATCGAAGGCTCTCTGCACGCCTCCTGAAAGAGAGAGAGAGAGAGAAGGAGAGAGAGATCATCACAGATCGATTCTCTCTTAAATCTCTCGTGAATCCCATTTCCCCTTCCGCTAGATTCTCTCTTCACTCATTTCTCGCTTTCTCTCCTTTTGTTCTCTCT 194 bp coding sequence for RNAi-2 AGGCGGAGATAGCCGCCAGGCTGCTGTCGCGCTTCAATCTGGTGGGCGGAGTCGGCGAAGGAGGCAGCTTACTAAATGCTCCTCTGCCTCTTCTCCTCCTCGATCCCCTTCCCCTCCTCCTACCATTCAAGCTCTTGTCTCTTCTTGTTTGGATTTTGGTCCTTGTACTCACTACTCCAACTCCAACTCCAACA

Table B. Primers used for gene detection Primer name Primer sequence UcFATB1 UcFATB1-F1 5’- GAGAGAGTGCACGAGGGATA -3’ UcFATB1-R1 5’- CTGCAGGAATTCCCGATCTA -3’ CsKASII CsKASII-F1uni 5’- TTGCTACAAGGCAACAGTGG -3’ CsKASII-R1uni 5’ - AGACCTTCATCCCTCCCATT -3’ PP2A PP2A-qF1 5’ – TAACGTCGCCAAAATGATGC -3’ PP2A-qR1 5’ – GTTCTCCACAACCGATTGGT -3’

β-phas CsKASII 169 AtFAD2 intron Nos RNAi -1 RNAi-2 Figure A. CsKASII RNAi constructs. RNAi-1, β-phas, β-phaseolin gene promoter; CsKASII 169, 169 bp 5’UTR sequence of a CsKASII. B: RNAi-2, Napin, Napin promoter; CsKASII 194, 194 bp of coding sequence of a CsKASII. AtFAD2 intron, linker sequence used between two reversely oriented DNA fragments; Nos, nopaline synthase gene terminator. See details in the text.

A. UcFATB1 Napin  mCherry 35S RB NOS LB BamH I Probe B. Figure B. Southern analysis. A. T-DNA section of UcFATB1 overexpression construct. RB: Right Border; Napin: Napin promoter; UcFATB1: California bay 12:0-acyl-carrier protein thioesterase gene (NCBI GI:170555); 35S: cauliflower mosaic virus 35S promoter; mCherry: mCherry fluorescence gene; LB: Left Border. BamHI was used for genomic DNA digestion and the probe used in Southern was a fragment of UcFATB1 coding sequence. B. Southern blot analysis of three independent UcFATB1 transgenic lines (No. 4, 12 and 75) and a non-transgenic camelina plant (Wt). MW

Figure C. Relative expression of CsKASII gene in CsKASII RNAi transformed and wild type lines. RNA was extracted from T3 individual immature seeds 15-20 days after pollination. Gene expression was measured by qRT-PCR SYBR Green method and normalized to PP2A expression.