1. Figure S3
Gui
3.9kb a TP b
Figure S3 Southern hybridization analysis of hygromycin resistant T1 plant lines.
a Southern hybridization analysis of hygromycin resistant Gui99 OsNPR1-RNAi T1 plant lines. The 422 bp BamHI/SpeI DNA fragment released from digestion of plasmid pCAMBIA1301-OsNPR1i was used as a probe. Gui99 is the untransformed control plant. 1-6, Gui99 transgenic lines I1, I2, I3, I5, I6, and I7, respectively.
b Southern hybridization analysis of hygromycin resistant TP309 OsNPR1-overexpressing T1 plant lines. The 3.1 kb EcoRV/XhoI DNA fragment (including partial OsNPR1 DNA) recovered from the digestion of plasmid pCAMBIA1301-UbiN-OsNPR1 was used as a probe. TP309 is the untransformed control plant; 1-8, TP309 transgenic lines 743, 758, 817, 878, 749, 862, 492, and 888, respectively.