1. P Xa13 :GUSP xa13 :GUS Supplemental Figure 1  -glucuronidase (GUS) expression regulated by the promoters of dominant Xa13 (P Xa13 ) and recessive xa13 (P xa13 ) genes when free of pathogen infection.

2. 0 h 3 d PXO99 Water P Xa13 :GFP P xa13 :GFP 0 h 3 d Supplemental Figure 2 Green fluorescent protein (GFP) expression regulated by the promoters of dominant Xa13 (P Xa13 ) and recessive xa13 (P xa13 ) genes. The leaves of transgenic plants were inoculated with Xoo strain PXO99 or water (control) immediately (0 h) or at 3 d after inoculation.

3. Supplemental Figure 3 Expression of dominant Xa13 and recessive xa13 by semiquantitative reverse transcription-PCR after inoculation with different Xoo strains, PXO99, PXO61, PXO79, and PXO145, in rice lines IRBB13 and IR24, respectively. IRBB13 and IR24 are near-isogenic lines. PXO61 PXO79 PXO99 PXO145 Xa13/xa13 Actin IRBB13 (xa13) IR24 (Xa13) 0 0.5 1 3 5 Time after inoculation of Xoo (day)

4. Xa13 rRNA IRBB13 (WT) T 0 plants (D129UI) transformed with P Ubi :Xa13 1 2 3 4 5 6 7 8 9 10 11 0 5 10 15 Lesion area (%) xa13 rRNA T 0 plants (D81UI) transformed with P Ubi :xa13 IRBB13 (WT) 1 2 3 4 5 6 7 8 9 10 11 12 13 0 5 10 15 Lesion area (%) xa13 Leu49 rRNA IRBB13 (WT) T 0 plants (D83UI) transformed with P Ubi :xa13 Leu49 1 2 3 4 5 6 7 8 9 10 11 12 35 36 37 38 0 5 10 15 Lesion area (%) Supplemental Figure 4 Transgenic plants constitutively expressing target genes. The plants were inoculated with Xoo strain PXO99. Bars represent mean (5 replicates)  standard deviation. The expression level of transgene was detected by RNA gel blot analysis using probe amplified with primers CDS4F (5’-AGTCGACGGGAGGGTACAG-3’) and CDS4R (5’- GTTCTGCAGCAGCTCCTTGT-3’).

5. Figure S4 (continued) Transgenic plants constitutively expressing target genes. The plants were inoculated with Xoo strain PXO99. Bars represent mean (5 replicates)  standard deviation. The expression level of transgene was detected by RNA gel blot analysis using probe amplified with primers CDS4F (5’-AGTCGACGGGAGGGTACAG-3’) and CDS4R (5’- GTTCTGCAGCAGCTCCTTGT-3’). 0 3 6 9 xa13 Val184 rRNA IRBB13 (WT) T 0 plants (D84UI) transformed with P Ubi :xa13 Val184 1 2 3 4 5 6 7 8 Lesion area (%) xa13 Ala85 rRNA IRBB13 (WT) T 0 plants (D82UI) transformed with P Ubi :xa13 Ala85 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 0 5 10 15 Lesion area (%)

6. 3 4 5 6 7 8 9 10 11 12 02468101214 Days after inoculation Bacteria [log (cfu/leaf)] IRBB13 (wild type) P Ubi :xa13 Leu49 :TNos (T 0 ) P Xa13 :xa13 Leu49 (T 0 ) IR24 Supplemental Figure 5 Growth of Xoo strain PXO99 in the leaves transgenic lines. cfu, colony-forming unit. IR24, the near-isogenic line of IRBB13, carrying dominant Xa13. Each data point represents mean (3 replicates)  standard deviation.

7. Supplemental Figure 6 Expression of pathogenesis-related genes PR1b/PR-1 and PR10/PBZ1 in near-isogenic lines IRBB13 carrying xa13 and IR24 carrying Xa13. Samples were collected at 0.5, 1, 3, and 5 d after inoculation with Xoo strain PXO61 or PXO99 or inoculated with water (control), which also served as wounding treatment for the inoculation was performed by leaf-clipping method. ck, without treatment. IRBB13 was resistant (incompatible) to PXO99 and susceptible (compatible) to PXO61, IR24 was susceptible (compatible) to PXO61 and PXO99. Bars represent mean (3 replicates)  standard deviation.