13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy

Slides:



Advertisements
Similar presentations
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
Advertisements

13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy.
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
Structure Determination: MS, IR, NMR (A review)
NMR Spectroscopy.
1 CHAPTER 13 Molecular Structure by Nuclear Magnetic Resonance (NMR)
Integration 10-6 Integration reveals the number of hydrogens responsible for an NMR peak. The area under an NMR peak is proportional to the number of equivalent.
1 Nuclear Magnetic Resonance Spectroscopy III Advanced Concepts: ORGANIC I LABORATORY W. J. Kelly.
Case Western Reserve University
Nuclear Magnetic Resonance (NMR) Spectroscopy
Chapter 13 Nuclear Magnetic Resonance Spectroscopy
1 Nuclear Magnetic Resonance Spectroscopy Renee Y. Becker Valencia Community College CHM 2011C.
Carbon-13 Nuclear Magnetic Resonance
Lecture 3 NMR Spectroscopy: Spin-spin Splitting in 1 H NMR Integration Coupling Constants 13 C NMR Sample Preparation for NMR Analysis Due: Lecture Problem.
NMR Theory and C-13 NMR. Nuclear Magnetic Resonance Powerful analysis – Identity – Purity No authentic needed Analyze nuclei – 1 H, 13 C, 31 P, etc –
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy Based on McMurry’s Organic Chemistry, 7 th edition.
Nuclear Magnetic Resonance (NMR) Spectroscopy Structure Determination
Nuclear Magnetic Resonance Spectroscopy. The Use of NMR Spectroscopy Used to map carbon-hydrogen framework of molecules Most helpful spectroscopic technique.
Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance
Nuclear Magnetic Resonance Spectroscopy Dr. Sheppard Chemistry 2412L.
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy Based on McMurry’s Organic Chemistry, 6 th edition.
1 Nuclear Magnetic Resonance Spectroscopy 13 C NMR 13 C Spectra are easier to analyze than 1 H spectra because the signals are not split. Each type of.
Structure Determination: Nuclear Magnetic Resonance Spectroscopy.
Chromatography (Separations) Mass Spectrometry Infrared (IR) Spectroscopy Nuclear Magnetic Resonance (NMR) Spectroscopy X-ray Crystallography (visual solid.
Nuclear Magnetic Resonance Spectroscopy. 2 Introduction NMR is the most powerful tool available for organic structure determination. It is used to study.
Chapter 14 NMR Spectroscopy Organic Chemistry 6th Edition Dr. Halligan
CHE 242 Unit V Structure and Reactions of Alcohols, Ethers and Epoxides; Basic Principles of NMR Spectroscopy CHAPTER THIRTEEN Terrence P. Sherlock Burlington.
Chapter 13 NMR Spectroscopy
Chapter 13 - Spectroscopy YSU 400 MHz Nuclear Magnetic Resonance Spectrometer(s)
Nuclear Magnetic Resonance Information Gained: Different chemical environments of nuclei being analyzed ( 1 H nuclei): chemical shift The number of nuclei.
Chapter 13 Structure Determination: Nuclear Magnetic Resonance Spectroscopy.
Important Concepts 10 1.NMR – Most important spectroscopic tool for elucidating organic structures. 2.Spectroscopy – Based on lower energy forms of molecules.
NMR Spectroscopy. NMR NMR uses energy in the radio frequency range. NMR uses energy in the radio frequency range. This energy is too low to cause changes.
The Number of Absorptions Protons have different chemical shifts when they are in different chemical environments Types of protons: – Homotopic Protons.
Nuclear Magnetic Resonance Spectroscopy
Structure Elucidation Method
NUCLEAR MAGNETIC RESONANCE SPECTROSCPY A guide for A level students KNOCKHARDY PUBLISHING.
MOLECULAR STRUCTURE ANALYSIS NMR Spectroscopy VCE Chemistry Unit 3: Chemical Pathways Area of Study 2 – Organic Chemistry.
© 2016 Cengage Learning. All Rights Reserved. John E. McMurry Chapter 13 Structure Determination: Nuclear Magnetic Resonance.
Integration10-6 Integration reveals the number of hydrogens responsible for an NMR peak. The area under an NMR peak is proportional to the number of equivalent.
11.1 Nuclear Magnetic Resonance Spectroscopy
The Use of NMR Spectroscopy
NMR spectroscopy – key principles
Department of chemistry Smt. K. R. P. Kanya Mahavidyalaya, Islampur
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
NMR Theory There are 2 variables in NMR: an applied magnetic field B0, and the frequency ( ) of radiation required for resonance, measured in MHz.
Nuclear Magnetic Resonance Spectroscopy
The Use of NMR Spectroscopy
Nuclear Magnetic Resonance Spectroscopy
Figure: 13.1 Title: Figure Nuclei in the absence and presence of an applied magnetic field. Caption: In the absence of an applied magnetic field,
13C NMR Spectroscopy Dr. A. G
Nuclear Magnetic Resonance Spectroscopy
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
Structure Determination: Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
A Summarized Look into…
Chapter 14 NMR: Connectivity
Chemical shift The relative energy of resonance of a particular nucleus resulting from its local environment is called chemical shift. NMR spectra show.
Nuclear Magnetic Resonance Spectroscopy
Advanced Pharmaceutical Analysis Nuclear Magnetic Resonance (H1 NMR)
Learning Objectives (13.1) Nuclear magnetic resonance spectroscopy
Introduction Nuclear magnetic resonance spectroscopy (NMR) is the most powerful tool available for organic structure determination. It is used to study.
Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance (NMR)
13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance (NMR)
Assis.Prof.Dr.Mohammed Hassan
The Use of NMR Spectroscopy
Presentation transcript:

13. Structure Determination: Nuclear Magnetic Resonance Spectroscopy Based on McMurry’s Organic Chemistry, 6th edition ©2003 Ronald Kluger Department of Chemistry University of Toronto

The Use of NMR Spectroscopy Used to determine relative location of atoms within a molecule Most helpful spectroscopic technique in organic chemistry Related to MRI in medicine (Magnetic Resonance Imaging) Maps carbon-hydrogen framework of molecules Depends on very strong magnetic fields McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.1 Nuclear Magnetic Resonance Spectroscopy 1H or 13C nucleus spins and the internal magnetic field aligns parallel to or against an aligned external magnetic field (See Figure 13.1) Parallel orientation is lower in energy making this spin state more populated Radio energy of exactly correct frequency (resonance) causes nuclei to flip into anti-parallel state Energy needed is related to molecular environment (proportional to field strength, B) – see Figure 13.2 McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.2 The Nature of NMR Absorptions Electrons in bonds shield nuclei from magnetic field Different signals appear for nuclei in different environments McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003 The NMR Measurement The sample is dissolved in a solvent that does not have a signal itself and placed in a long thin tube The tube is placed within the gap of a magnet and spun Radiofrequency energy is transmitted and absorption is detected Species that interconvert give an averaged signal that can be analyzed to find the rate of conversion McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003 13.3 Chemical Shifts The relative energy of resonance of a particular nucleus resulting from its local environment is called chemical shift NMR spectra show applied field strength increasing from left to right Left part is downfield is upfield Nuclei that absorb on upfield side are strongly shielded. Chart calibrated versus a reference point, set as 0, tetramethylsilane [TMS] McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

Measuring Chemical Shift Numeric value of chemical shift: difference between strength of magnetic field at which the observed nucleus resonates and field strength for resonance of a reference Difference is very small but can be accurately measured Taken as a ratio to the total field and multiplied by 106 so the shift is in parts per million (ppm) Absorptions normally occur downfield of TMS, to the left on the chart Calibrated on relative scale in delta () scale  is the number of parts per million (ppm) of the magnetic field expressed as the spectrometer’s operating frequency (used ahead of value as it is a ratio and not a unit) Independent of instrument’s field strength McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.4 13C NMR Spectroscopy: Signal Averaging and FT-NMR Carbon-13: only carbon isotope with a nuclear spin Natural abundance 1.1% of C’s in molecules Sample is thus very dilute in this isotope Sample is measured using repeated accumulation of data and averaging of signals, incorporating pulse and the operation of Fourier transform (FT-NMR) All signals are obtained simultaneously using a broad pulse of energy and resonance recorded Frequent repeated pulses give many sets of data that are averaged to eliminate noise Fourier-transform of averaged pulsed data gives spectrum (see Figure 13-6) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.5 Characteristics of 13C NMR Spectroscopy Provides a count of the different types of environments of carbon atoms in a molecule 13C resonances are 0 to 220 ppm downfield from TMS (Figure 13-7) Chemical shift affected by electronegativity of nearby atoms O, N, halogen decrease electron density and shielding (“deshield”), moving signal downfield. sp3 C signal is at  0 to 9; sp2 C:  110 to 220 C(=O) at the low field,  160 to 220 Spectrum of 2-butanone is illustrative- signal for C=O carbons on left edge Read about para-bromoacetophenone (Figure 13-8 b). McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.6 DEPT 13C NMR Spectroscopy Improved pulsing and computational methods give additional information DEPT-NMR (distortionless enhancement by polarization transfer) Normal spectrum shows all C’s then: Obtain spectrum of all C’s except quaternary (broad band decoupled) Change pulses to obtain separate information for CH2, CH Subtraction reveals each type (See Figure 13-10) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.7 Uses of13C NMR Spectroscopy Provides details of structure Example: product orientation in elimination from 1-chloro-methyl cyclohexane Difference in symmetry of products is directly observed in the spectrum 1-Methylcyclohexene has five sp3 resonances ( 20-50) and two sp2 resonances  100-150 (see Figure13-11) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.8 1H NMR Spectroscopy and Proton Equivalence Proton NMR is much more sensitive than 13C and the active nucleus (1H) is nearly 100 % of the natural abundance Shows how many kinds of nonequivalent hydrogens are in a compound Theoretical equivalence can be predicted by seeing if replacing each H with “X” gives the same or different outcome Equivalent H’s have the same signal while nonequivalent are different There are degrees of nonequivalence McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003 Nonequivalent H’s Replacement of each H with “X” gives a different constitutional isomer Then the H’s are in constitutionally heterotopic environments and will have different chemical shifts – they are nonequivalent under all circumstances McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003 Equivalent H’s Two H’s that are in identical environments (homotopic) have the same NMR signal Test by replacing each with X if they give the identical result, they are equivalent McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

Enantiotopic Distinctions If H’s are in environments that are mirror images of each other, they are enantiotopic Replacement of each H with X produces a set of enantiomers The H’s have the same NMR signal (in the absence of chiral materials) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

Diastereotopic Distinctions In a chiral molecule, paired hydrogens can have different environments and different shifts Replacement of a pro-R hydrogen with X gives a different diastereomer than replacement of the pro-S hydrogen Diastereotopic hydrogens are distinct chemically and spectrocopically McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.9 Chemical Shifts in 1H NMR Spectroscopy Proton signals range from  0 to  10 Lower field signals are H’s attached to sp2 C Higher field signals are H’s attached to sp3 C Electronegative atoms attached to adjacent C cause downfield shift See Tables 13-2 and 13-3 for a complete list McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.10 Integration of 1H NMR Absorptions: Proton Counting The relative intensity of a signal (integrated area) is proportional to the number of protons causing the signal This information is used to deduce the structure For example in ethanol (CH3CH2OH), the signals have the integrated ratio 3:2:1 For narrow peaks, the heights are the same as the areas and can be measured with a ruler McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.11 Spin-Spin Splitting in 1H NMR Spectra Peaks are often split into multiple peaks due to interactions between nonequivalent protons on adjacent carbons, called spin-spin splitting The splitting is into one more peak than the number of H’s on the adjacent carbon (“n+1 rule”) The relative intensities are in proportion of a binomial distribution and are due to interactions between nuclear spins that can have two possible alignments with respect to the magnetic field The set of peaks is a multiplet (2 = doublet, 3 = triplet, 4 = quartet) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

Simple Spin-Spin Splitting An adjacent CH3 group can have four different spin alignments as 1:3:3:1 This gives peaks in ratio of the adjacent H signal An adjacent CH2 gives a ratio of 1:2:1 The separation of peaks in a multiplet is measured is a constant, in Hz J (coupling constant) McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

Rules for Spin-Spin Splitting Equivalent protons do not split each other The signal of a proton with n equivalent neighboring H’s is split into n + 1 peaks Protons that are farther than two carbon atoms apart do not split each other McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.12 More Complex Spin-Spin Splitting Patterns Spectra can be more complex due to overlapping signals, multiple nonequivalence Example: trans-cinnamaldehyde McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003

13.13 Uses of 1H NMR Spectroscopy The technique is used to identify likely products in the laboratory quickly and easily Example: regiochemistry of hydroboration/oxidation of methylenecyclohexane Only that for cyclohexylmethanol is observed McMurry Organic Chemistry 6th edition Chapter 13 (c) 2003