1. Department of chemistry Smt. K. R. P. Kanya Mahavidyalaya, Islampur
WEL-COME
Dr. Sambhaji Rajaram Mane
M. Sc. B.Ed. Ph.D.
Associate Professor and Head
Department of chemistry
Smt. K. R. P. Kanya Mahavidyalaya, Islampur

2. Carbon 13 NMR Spectroscopy

3. Theory of NMR
The positively charged nuclei of certain elements (e.g., 13C and 1H) behave as tiny magnets.
In the presence of a strong external magnetic field (Bo), these nuclear magnets align either with ( ) the applied field or opposed to ( ) the applied field.
The latter (opposed) is slightly higher in energy than aligned with the field.
DE is very small

4. Theory of NMR
The small energy difference between the two alignments of magnetic spin corresponds to the energy of radio waves according to Einstein’s equation E=hn.
Application of just the right radiofrequency (n) causes the nucleus to “flip” to the higher energy spin state
Not all nuclei require the same amount of energy for the quantized spin ‘flip’ to take place.
The exact amount of energy required depends on the chemical identity (H, C, or other element) and the chemical environment of the particular nucleus.

5. Theory of NMR
Our department’s NMR spectrometer (in Dobo 245) has a superconducting magnet with a field strength of 9.4 Tesla. On this instrument, 1H nuclei absorb (resonate) near a radiofrequency of 400 MHz; C nuclei absorb around 100 MHz.
Nuclei are surrounded by electrons The strong applied magnetic field (Bo) induces the electrons to circulate around the nucleus (left hand rule).
(9.4 T)

6. Theory of NMR
The induced circulation of electrons sets up a secondary (induced) magnetic field (Bi) that opposes the applied field (Bo) at the nucleus (right hand rule).
We say that nuclei are shielded from the full applied magnetic field by the surrounding electrons because the secondary field diminishes the field at the nuclei.

7. Theory of NMR
The electron density surrounding a given nucleus depends on the electronegativity of the attached atoms.
The more electronegative the attached atoms, the less the electron density around the nucleus in question.
We say that that nucleus is less shielded, or is deshielded by the electronegative atoms.
Deshielding effects are generally additive. That is, two highly electronegative atoms (2 Cl atoms, for example) would cause more deshielding than only 1 Cl atom.
C and H are deshielded C and H are more deshielded

8. Proton Decoupling 13C H 25 MHz 100 MHz (in frame rotating at 100 MHz)
irradiate H (100 MHz) and pulse (25MHz)
to observe C13
13C H
C13 NMR
spectrum
H NMR
spectrum
C13 up
C13 down
C12 or or
H up
H down
J ~ 125 Hz
H average

9. Chemical Shift
We call the relative position of absorption in the NMR spectrum (which is related to the amount of deshielding) the chemical shift. It is a unitless number (actually a ratio, in which the units cancel), but we assign ‘units’ of ppm or d (Greek letter delta) units.
For 1H, the usual scale of NMR spectra is 0 to 10 (or 12) ppm (or d).
The usual 13C scale goes from 0 to about 220 ppm.
The zero point is defined as the position of absorption of a standard, tetramethylsilane (TMS):
This standard has only one type of C and only one type of H.

10. Chemical Shifts

11. Chemical Shifts
Both 1H and 13C Chemical shifts are related to three major factors:
The hybridization (of carbon)
Presence of electronegative atoms or electron attracting groups
The degree of substitution (1º, 2º or 3º). These latter effects are most important in 13C NMR, and in that context are usually called ‘steric’ effects.
First we’ll focus on Carbon NMR spectra
(they are simpler)

12. CMR Spectra
Each unique C in a structure gives a single peak in the spectrum; there is rarely any overlap.
The carbon spectrum spans over 200 ppm; chemical shifts only ppm apart can be distinguished; this allows for over 2x105 possible chemical shifts for carbon.
The intensity (size) of each peak is NOT directly related to the number of that type of carbon. Other factors contribute to the size of a peak:
Peaks from carbon atoms that have attached hydrogen atoms are bigger than those that don’t have hydrogens attached.
Carbon chemical shifts are usually reported as downfield from the carbon signal of tetramethylsilane (TMS).

13. 13C Chemical Shifts

14. 1H and 13C NMR compared:
both give us information about the number of chemically nonequivalent nuclei (nonequivalent hydrogens or nonequivalent carbons)
both give us information about the environment of the nuclei (hybridization state, attached atoms, etc.) 6

15. 1H and 13C NMR compared:
the signal for the NMR of a 13 C nucleus is 10-4 times weaker than the signal for a hydrogen nucleus
a signal for a 13C nucleus is only about 1% as intense as that for 1H because of the magnetic properties of the nuclei, and
at the "natural abundance" level only 1.1% of all the C atoms in a sample are 13C (most are 12C) 6

16. 1H and 13C NMR compared:
13C signals are spread over a much wider range than 1H signals making it easier to identify and count individual nuclei
Check the spectra on the next slides: Figure (a) shows the 1H NMR spectrum of 1-chloropentane; Figure (b) shows the 13C spectrum. It is much easier to identify the compound as 1-chloropentane by its 13C spectrum than by its 1H spectrum. 6

17. 1H Figure 13.20(a) (page 511) Chemical shift (d, ppm) 1 ClCH2 CH3
ClCH2CH2CH2CH2CH3
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
Chemical shift (d, ppm) 1

18. 13C a separate, distinct peak appears for each of the 5 carbons CDCl3
Figure 13.20(b) (page 511) Note: in these spectra the peak intensities are not exactly proportional to the number of carbon atoms.
13C
ClCH2CH2CH2CH2CH3
a separate, distinct peak appears for each of the 5 carbons
CDCl3 20 40 60 80
100
120
140
160
180
200
Chemical shift (d, ppm) 1

19. are measured in ppm (d) from the carbons of TMS
C Chemical Shifts
are measured in ppm (d) from the carbons of TMS 3

20. 13C Chemical shifts are most affected by:
hybridization state of carbon
electronegativity of groups attached to carbon 6

21. Examples (chemical shifts in ppm from TMS)23
138
sp3 hybridized carbon is more shielded than sp2 6

22. Examples (chemical shifts in ppm from TMS)OH 61 O
202
sp3 hybridized carbon is more shielded than sp2 6

23. Examples (chemical shifts in ppm from TMS)OH 23 61
an electronegative atom deshields the carbon to which it is attached 6

24. Examples (chemical shifts in ppm from TMS)
138
202
an electronegative atom deshields the carbon to which it is attached 6

25. Table 13.3 (p 513) Type of carbon Chemical shift (d), ppm 25 RCH3 0-35
15-40
R3CH
25-50
R4C
30-40 25

26. Table 13.3 (p 513) Type of carbon Chemical shift (d), ppm
RCH3
0-35 CR RC
65-90
R2CH2
15-40
CR2
R2C
R3CH
25-50
R4C
30-40 25

27. Table 13.3 (p 513) Type of carbon Chemical shift (d), ppm 25 RCH2Br
20-40
RCH2Cl
25-50
RCH2NH2
35-50
RCH2OH
50-65
RCH2OR
50-65 25

28. Table 13.3 (p 513) Type of carbon Chemical shift (d), ppm
RCH2Br
20-40
RCOR
RCH2Cl
25-50
RCH2NH2
35-50 O
RCH2OH
50-65
RCR
RCH2OR
50-65 25

29. Predicting 13C Spectra
Problem Predict the number of carbon resonance lines in the 13C spectra of the following (= # unique Cs):
4 lines
plane of symmetry

30. Predicting 13C Spectra
Predicte the number of carbon resonance lines in the 13C spectra of the major product of the following reaction:
7 lines
5 lines
plane of symmetry

31. Predicting 13C Spectra

36. C6H12O2

45. DEPT 13C NMR distinguish among CH3, CH2, and CH
groups

46. THANK FOR YOUR ATTENTION46