HPTS- Optical pH Sensor pH sensitive fluorescent dye Absorbance / Excitation changes with pH Emission at 520 nm Excitation Ratiometric
pH SENSOR ASSEMBLY FOR OPTICAL CHEMICAL SENSOR White microfiltration membrane backing PEG-dye copolymer hydrogel Transfer adhesive Note: All Optical Chemical Sensors Are Based on Same Concept as pH Sensor
pH Sensor Calibration in Cell Suspension
E.coli Shake Flask Fermentation with pH Coaster and Sensor Patch Conditions: LB Media, T=37°C, 300 rpm Discrepancy at 6 hrs+ likely due to CO 2
Measurement of pCO 2 Important metabolite Critical in bacterial and mammalian cell cultures Conventional technology uses pH probe inside bicarbonate buffer- cumbersome New optical alternative
APPLICATION OF OPTICAL CHEMICAL CO 2 SENSOR IN FERMENTATION: E. COLI OD CO 2
DISSOLVED OXYGEN OPTICAL- CHEMICAL SENSOR CONSTRUCTION
On-line turbidity measurement in E.coli fermentation