Development of Personalized Tumor Biomarkers Using Massively Parallel Sequencing by Rebecca J. Leary, Isaac Kinde, Frank Diehl, Kerstin Schmidt, Chris.

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Development of Personalized Tumor Biomarkers Using Massively Parallel Sequencing by Rebecca J. Leary, Isaac Kinde, Frank Diehl, Kerstin Schmidt, Chris Clouser, Cisilya Duncan, Alena Antipova, Clarence Lee, Kevin McKernan, Francisco M. De La Vega, Kenneth W. Kinzler, Bert Vogelstein, Luis A. Diaz, and Victor E. Velculescu Sci Transl Med Volume 2(20):20ra14-20ra14 February 24, 2010 Published by AAAS

Fig. 1. Schematic of PARE approach. Rebecca J. Leary et al., Sci Transl Med 2010;2:20ra14 Published by AAAS

Fig. 2. Detection of tumor-specific rearrangements in breast and colorectal cancers. Rebecca J. Leary et al., Sci Transl Med 2010;2:20ra14 Published by AAAS

Fig. 3. Detection of tumor-specific rearrangements in mixtures of tumor and normal DNA. Decreasing amounts of tumor DNA were mixed with increasing amounts of normal tissue DNA (300 ng total) and were used as template molecules for PCR using chromosome 4:8 translocation-specific primers or chromosome 3 con Rebecca J. Leary et al., Sci Transl Med 2010;2:20ra14 Published by AAAS

Fig. 4. Detection of tumor-specific rearrangements in plasma of cancer patients. Rebecca J. Leary et al., Sci Transl Med 2010;2:20ra14 Published by AAAS