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Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion,

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Presentation on theme: "Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion,"— Presentation transcript:

1 Productive Replication of Ebola Virus Is Regulated by the c-Abl1 Tyrosine Kinase by Mayra García, Arik Cooper, Wei Shi, William Bornmann, Ricardo Carrion, Daniel Kalman, and Gary J. Nabel Sci Transl Med Volume 4(123):123ra24-123ra24 February 29, 2012 Published by AAAS

2 Fig. 1 Effect of c-Abl1 knockdown and kinase inhibition on Ebola VLP release in transfected 293T cells. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

3 Fig. 2 Toxicity analysis, morphology of Ebola intracellular nucleocapsids, and nucleocapsid formation. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

4 Fig. 3 c-Abl1–specific siRNA and nilotinib effects on VP40 VLP egress. 293T cells were transfected with plasmid encoding VP40. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

5 Fig. 4 Effect of c-Abl1 expression on VP40 phosphorylation and sensitivity to TK antagonist inhibition and siRNA. (A) Western analysis of transfected cell lysates (lanes 1 to 4) or VP40 immunoprecipitates (IP) (lanes 5 to 8) of cells transfected with empty vector control (lanes 1, 3, 5, and 7) or wild-ty Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

6 Fig. 5 Localization of VP40 c-Abl1–mediated tyrosine phosphorylation and effect of tyrosine mutation on VLP release. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS

7 Fig. 6 Effect of siRNA knockdown and c-Abl1 TK inhibition on Ebola virus replication. Mayra García et al., Sci Transl Med 2012;4:123ra24 Published by AAAS


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